REL-1017 (esmethadone; D-methadone) does not cause reinforcing effect, physical dependence and withdrawal signs in Sprague Dawley rats.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
06 07 2022
Historique:
received: 22 11 2021
accepted: 17 05 2022
entrez: 6 7 2022
pubmed: 7 7 2022
medline: 9 7 2022
Statut: epublish

Résumé

REL-1017 (esmethadone, D-methadone) is the opioid-inactive d-isomer of racemic D,L-methadone. REL-1017 may exert antidepressant effects via uncompetitive N-methyl-D-aspartate receptor (NMDAR) channel block. As REL-1017 is expected to exert central nervous system activity, full characterization of its abuse potential is warranted. We evaluated lack of reinforcing effect, physical dependence, and withdrawal of REL-1017 in Sprague Dawley rats. (1) Self-administration Study Rats were trained to self-administer oxycodone intravenously (IV) and then were subjected to 3-day substitution tests where saline, oxycodone, and REL-1017 were self-delivered IV by a fixed number of lever presses; (2) Drug Discontinuation Study Rats were treated for 30 days by oral gavage with vehicle, REL-1017, ketamine or morphine and evaluated for withdrawal with functional observational batteries (FOBs). In the self-administration study, rats treated with saline, vehicle, and all REL-1017 doses showed the typical "extinction burst" pattern of response, characterized by an initial rapid increase of lever-pressing followed by a rapid decrease over 3 days. Rats treated with oxycodone maintained stable self-injection, as expected for reinforcing stimuli. In the withdrawal study, REL-1017 did not engender either morphine or ketamine withdrawal signs over 9 days following abrupt discontinuation of drug exposure. REL-1017 showed no evidence of abuse potential and did not engender withdrawal symptomatology.

Identifiants

pubmed: 35794162
doi: 10.1038/s41598-022-15055-3
pii: 10.1038/s41598-022-15055-3
pmc: PMC9259683
doi:

Substances chimiques

D-methadone 0
Ketamine 690G0D6V8H
Morphine 76I7G6D29C
Oxycodone CD35PMG570
Methadone UC6VBE7V1Z

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11389

Informations de copyright

© 2022. The Author(s).

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Auteurs

Jack Henningfield (J)

Pinney Associates, Bethesda, MA, USA.

David Gauvin (D)

Charles River Laboratories, Wilmington, MA, USA.

Francesco Bifari (F)

Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.

Reginald Fant (R)

Pinney Associates, Bethesda, MA, USA.

Megan Shram (M)

Altreos Research Partners, Toronto, ON, Canada.

August Buchhalter (A)

Pinney Associates, Bethesda, MA, USA.

Judy Ashworth (J)

Pinney Associates, Bethesda, MA, USA.

Ryan Lanier (R)

Pinney Associates, Bethesda, MA, USA.

Marco Pappagallo (M)

Relmada Therapeutics, Inc., Coral Gables, FL, USA.

Charles Inturrisi (C)

Relmada Therapeutics, Inc., Coral Gables, FL, USA.

Franco Folli (F)

Department of Health Sciences, University of Milan, Milan, Italy.

Sergio Traversa (S)

Relmada Therapeutics, Inc., Coral Gables, FL, USA.

Paolo L Manfredi (PL)

Relmada Therapeutics, Inc., Coral Gables, FL, USA. PManfredi@relmada.com.

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Classifications MeSH