Oxytocin via oxytocin receptor excites neurons in the endopiriform nucleus of juvenile mice.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 07 2022
06 07 2022
Historique:
received:
24
01
2022
accepted:
23
06
2022
entrez:
6
7
2022
pubmed:
7
7
2022
medline:
9
7
2022
Statut:
epublish
Résumé
The neuropeptide oxytocin (OXT) modulates social behaviors across species and may play a developmental role for these behaviors and their mediating neural pathways. Despite having high, stable levels of OXT receptor (OXTR) ligand binding from birth, endopiriform nucleus (EPN) remains understudied. EPN integrates olfactory and gustatory input and has reciprocal connections with several limbic areas. Because the role of OXTR signaling in EPN is unknown, we sought to provide anatomical and electrophysiological information about OXTR signaling in mouse EPN neurons. Using in situ hybridization, we found that most EPN neurons co-express Oxtr mRNA and the marker for VGLUT1, a marker for glutamatergic cells. Based on high levels of OXTR ligand binding in EPN, we hypothesized that oxytocin application would modulate activity in these cells as measured by whole-cell patch-clamp electrophysiology. Bath application of OXT and an OXTR specific ligand (TGOT) increased the excitability of EPN neurons in wild-type, but not in OXTR-knockout (KO) tissue. These results show an effect of OXT on a mainly VGLUT1+ cell population within EPN. Given the robust, relatively stable OXTR expression in EPN throughout life, OXTR in this multi-sensory and limbic integration area may be important for modulating activity in response to an array of social or other salient stimuli throughout the lifespan and warrants further study.
Identifiants
pubmed: 35794163
doi: 10.1038/s41598-022-15390-5
pii: 10.1038/s41598-022-15390-5
pmc: PMC9259672
doi:
Substances chimiques
Ligands
0
OXTR protein, mouse
0
Receptors, Oxytocin
0
Oxytocin
50-56-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
11401Subventions
Organisme : NIH HHS
ID : MH114994
Pays : United States
Informations de copyright
© 2022. The Author(s).
Références
J Neurosci. 2018 Jan 31;38(5):1218-1231
pubmed: 29279308
Nature. 2015 Sep 24;525(7570):519-22
pubmed: 26375004
J Pharmacol Exp Ther. 2013 Aug;346(2):318-27
pubmed: 23723434
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16096-101
pubmed: 16249339
PLoS One. 2017 Feb 24;12(2):e0172904
pubmed: 28235051
Neuron. 2018 Sep 5;99(5):887-904
pubmed: 30189208
PLoS One. 2008 Jul 16;3(7):e2695
pubmed: 18628994
Front Behav Neurosci. 2013 Dec 11;7:195
pubmed: 24376405
J Neurosci. 2020 Mar 11;40(11):2282-2295
pubmed: 32024781
Int J Neuropsychopharmacol. 2017 Oct 1;20(10):861-866
pubmed: 28977526
Biochem Biophys Res Commun. 2020 Jul 12;528(1):174-178
pubmed: 32482389
Neuron. 2012 Feb 9;73(3):553-66
pubmed: 22325206
Nat Neurosci. 2004 Oct;7(10):1048-54
pubmed: 15452576
Biol Psychiatry. 2014 Sep 1;76(5):377-86
pubmed: 24787950
Neurosci Biobehav Rev. 1987 Winter;11(4):365-89
pubmed: 2830564
Neuron. 2018 Nov 7;100(3):593-608.e3
pubmed: 30293821
Nat Commun. 2020 Apr 20;11(1):1885
pubmed: 32313029
J Physiol. 1994 Jul 15;478 ( Pt 2):275-87
pubmed: 7525943
Dev Neurobiol. 2017 Feb;77(2):143-157
pubmed: 27273834
J Neurosci. 2009 Feb 18;29(7):2259-71
pubmed: 19228979
Physiol Behav. 2004 Nov 15;83(2):319-28
pubmed: 15488548
Psychoneuroendocrinology. 2015 Dec;62:36-46
pubmed: 26231445
Neurosci Lett. 2001 Feb 16;299(1-2):65-8
pubmed: 11166939
J Neurophysiol. 2020 Feb 1;123(2):587-599
pubmed: 31891523
Nat Genet. 2000 Jul;25(3):284-8
pubmed: 10888874
Eur J Neurosci. 2016 Dec;44(11):2885-2898
pubmed: 27717106
Neuron. 2015 Jul 1;87(1):152-63
pubmed: 26139372
J Comp Neurol. 1978 Mar 15;178(2):255-80
pubmed: 627626
Horm Behav. 2020 Feb;118:104613
pubmed: 31654673
J Neurosci. 2016 Feb 24;36(8):2517-35
pubmed: 26911697
Psychopharmacology (Berl). 2013 Oct;229(4):639-51
pubmed: 23624810
Nature. 2013 Aug 22;500(7463):458-62
pubmed: 23913275
Nat Commun. 2017 Dec 8;8(1):2001
pubmed: 29222469
Nature. 2013 Sep 12;501(7466):179-84
pubmed: 24025838
Neurosci Res. 2002 Apr;42(4):243-50
pubmed: 11985876
Neural Plast. 2018 Jul 2;2018:4864107
pubmed: 30057594
Chem Senses. 2012 Oct;37(8):681-8
pubmed: 22490644
Neurosci Res. 2015 Jan;90:90-4
pubmed: 24929102
J Comp Neurol. 1999 Jun 14;408(4):532-48
pubmed: 10340503
Cell. 2014 Oct 9;159(2):295-305
pubmed: 25303526
Horm Behav. 2016 Jan;77:249-59
pubmed: 26122293
Biol Psychiatry. 2011 May 1;69(9):875-82
pubmed: 21306704
Neuroscience. 2004;126(4):1033-41
pubmed: 15207336
J Neurosci. 2001 Oct 15;21(20):8278-85
pubmed: 11588199
Neuron. 2016 May 4;90(3):609-21
pubmed: 27112498
Elife. 2017 Dec 12;6:
pubmed: 29231812
Nat Rev Neurosci. 2006 Feb;7(2):126-36
pubmed: 16429122
Brain Res. 1996 Sep 16;733(2):292-6
pubmed: 8891313
Trends Pharmacol Sci. 1998 Dec;19(12):500-5
pubmed: 9871412
Nature. 2003 Oct 30;425(6961):917-25
pubmed: 14586460
Front Neuroendocrinol. 2018 Oct;51:14-24
pubmed: 29054552
Nat Neurosci. 2003 Apr;6(4):384-90
pubmed: 12598900
Cell Signal. 2017 Sep;37:93-102
pubmed: 28603013
Peptides. 2004 Sep;25(9):1565-74
pubmed: 15374658
Psychoneuroendocrinology. 2014 Dec;50:14-9
pubmed: 25173438
Sci Rep. 2020 Nov 17;10(1):19943
pubmed: 33203885
Neurosci Lett. 2008 Jun 20;438(2):133-7
pubmed: 18468792
Prog Brain Res. 2008;170:513-7
pubmed: 18655904
Neuron. 2008 Jun 26;58(6):911-24
pubmed: 18579081
Trends Neurosci. 2004 Feb;27(2):98-103
pubmed: 15102489
Brain Res Bull. 2002 Jun;58(2):141-7
pubmed: 12127011
Psychoneuroendocrinology. 2016 Jun;68:156-62
pubmed: 26974500
J Neurochem. 2010 Sep 1;114(5):1424-35
pubmed: 20557424
Psychoneuroendocrinology. 2021 Jan;123:105044
pubmed: 33227537
J Neurosci. 2012 Oct 31;32(44):15476-88
pubmed: 23115185
Curr Biol. 2021 Jan 25;31(2):322-333.e5
pubmed: 33157028
Nature. 2015 Apr 23;520(7548):499-504
pubmed: 25874674
Proc Natl Acad Sci U S A. 2022 Mar 8;119(10):e2120093119
pubmed: 35238656
Mol Pain. 2008 May 29;4:19
pubmed: 18510735
Pain. 2009 Aug;144(3):320-328
pubmed: 19493620
J Neuroendocrinol. 2006 Sep;18(9):685-91
pubmed: 16879167
J Neurosci. 2014 Jun 18;34(25):8499-506
pubmed: 24948805