Bombyx mori Vps13d is a key gene affecting silk yield.
Animals
Bombyx
/ chemistry
Drosophila Proteins
Drosophila melanogaster
/ chemistry
Female
Genes, Insect
/ genetics
Humans
Insect Proteins
/ genetics
Intracellular Signaling Peptides and Proteins
Larva
/ anatomy & histology
Male
Mutation
Promoter Regions, Genetic
/ genetics
Proteins
Silk
/ biosynthesis
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
26
04
2022
accepted:
20
06
2022
entrez:
7
7
2022
pubmed:
8
7
2022
medline:
12
7
2022
Statut:
epublish
Résumé
Bombyx mori is an important economic insect, its economic value mainly reflected in the silk yield. The major functional genes affecting the silk yield of B. mori have not been determined yet. Bombyx mori vacuolar protein sorting-associated protein 13d (BmVps13d) has been identified, but its function is not reported. In this study, BmVps13d protein shared 30.84% and 34.35% identity with that of in Drosophila melanogaster and Homo. sapiens, respectively. The expressions of BmVps13d were significantly higher in the midgut and silk gland of JS (high silk yield) than in that of L10 (low silk yield). An insertion of 9 bp nucleotides and two deficiencies of adenine ribonucleotides in the putative promoter region of BmVps13d gene in L10 resulted in the decline of promoter activity was confirmed using dual luciferase assay. Finally, the functions of BmVps13d in B. mori were studied using the CRISPR/Cas9 system, and the mutation of BmVps13d resulted in a 24.7% decline in weight of larvae, as well as a 27.1% (female) decline and a 11.8% (male) decline in the silk yield. This study provides a foundation for studying the molecular mechanism of silk yield and breeding the silkworm with high silk yield.
Identifiants
pubmed: 35797274
doi: 10.1371/journal.pone.0270840
pii: PONE-D-22-12066
pmc: PMC9262180
doi:
Substances chimiques
Drosophila Proteins
0
Insect Proteins
0
Intracellular Signaling Peptides and Proteins
0
Proteins
0
Silk
0
VPS13D protein, human
0
Vps13D protein, Drosophila
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0270840Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
Références
Development. 2013 Dec;140(24):4982-7
pubmed: 24257628
Dev Cell. 2011 Jul 19;21(1):77-91
pubmed: 21763610
Bioinformatics. 2015 Apr 1;31(7):1120-3
pubmed: 25414360
Curr Biol. 2018 Jan 22;28(2):R66-R68
pubmed: 29374445
Traffic. 2011 Oct;12(10):1318-26
pubmed: 21722282
Sci Rep. 2020 Mar 30;10(1):5682
pubmed: 32231221
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6766-70
pubmed: 23569267
Cell Host Microbe. 2013 Sep 11;14(3):232-41
pubmed: 24034610
Insect Sci. 2020 Feb;27(1):2-13
pubmed: 29943911
Nat Ecol Evol. 2018 Aug;2(8):1268-1279
pubmed: 29967484
Insect Mol Biol. 2019 Oct;28(5):728-738
pubmed: 30955208
Front Genet. 2020 Mar 27;11:225
pubmed: 32292415
Curr Biol. 2018 Jan 22;28(2):287-295.e6
pubmed: 29307555
Crit Rev Biochem Mol Biol. 2010 Dec;45(6):463-87
pubmed: 20653365
BMC Genomics. 2015 Feb 06;16:60
pubmed: 25887670
Nat Rev Mol Cell Biol. 2010 Aug;11(8):556-66
pubmed: 20588296
Tissue Cell. 2010 Feb;42(1):42-6
pubmed: 19665160
Nat Commun. 2021 Feb 23;12(1):1252
pubmed: 33623047
Nat Cell Biol. 2011 Dec 22;14(1):38-45
pubmed: 22193162
Annu Rev Entomol. 2014;59:513-36
pubmed: 24160415
J Cell Biol. 2021 May 3;220(5):
pubmed: 33891012
Nature. 2009 Mar 26;458(7237):445-52
pubmed: 19325624
Int J Mol Sci. 2018 Nov 22;19(12):
pubmed: 30467288
Insect Biochem Mol Biol. 2015 Jul;62:211-25
pubmed: 25486452