Keratin5-cytoskeleton-BMP4 network regulates cell phenotype conversions during cardiac regeneration.

Animals Bone Morphogenetic Protein 4 Cytoskeleton / metabolism Mechanotransduction, Cellular Myocytes, Cardiac / metabolism Phenotype Zebrafish / metabolism Zebrafish Proteins / genetics
BMP signaling Blastema regeneration Keratin Mechanotransduction Myocardium resalvage Zebrafish

Journal

Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226

Informations de publication

Date de publication:
01 09 2022
Historique:
received: 03 12 2021
revised: 26 06 2022
accepted: 30 06 2022
pubmed: 8 7 2022
medline: 20 7 2022
entrez: 7 7 2022
Statut: ppublish

Résumé

The reconstruction of a blood supply system and myocardial recovery from inflamamtory reactions in the infract zone remains a challenge in cardiac regeneration after myocardial infarction. Here, we observed that the local myocardial cells and the clotted blood cells undergo cellular remodeling via cytoplasmic exocytosis and nuclear reorganization in zebrafish hearts after resection of the ventricular apex. The subsequent tissue regeneration processes were visualized by detection of the spatiotemporal expression of three tissue specific genes (α-SMA which marks for vasculature/fibrogenesis, Flk1for angiogenesis/hematopoiesis, and Pax3a for remusculogensis), and two histone modification markers (H3K9Ac and H3K9Me3 for chromatin remodeling). By analyzing the composition of the blastema tissue fractions we found that Krt5 peptide could promote F-actin assembly, BMP4-pSmad2/5/8 signaling activity, and H3K9Me3-mediated chromatin accessibility at the blastema representative genes in the cultured zebrafish embryonic fibroblasts. Further in vivo tests demonstrated that Krt5 interacted with beta actin, and promoted Gata3 expression and Flk1-GFP marked blastema angiogenesis. These results proposed a new Krt5-cytoskeleton-BMP4 mechanotransduction mechanism in the epithelial-dependent and cell phenotype conversion-based tissue regeneration.

Identifiants

DOI: 10.1016/j.yexcr.2022.113272 PMID: 35798073
pubmed: 35798073
pii: S0014-4827(22)00265-8
doi: 10.1016/j.yexcr.2022.113272
pii:
doi:

Substances chimiques

Bone Morphogenetic Protein 4 0
Zebrafish Proteins 0
bmp4 protein, zebrafish 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113272

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Xuelong Wang (X)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China; CAS Key Laboratory of Computational Biology, Chinese Academy of Sciences, Shanghai, China.

Huiping Guo (H)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China.

Feifei Yu (F)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China.

Hui Zhang (H)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China.

Ying Peng (Y)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China.

Chenghui Wang (C)

Department of Aquaculture, Shanghai Ocean University, Shanghai, China.

Gang Wei (G)

CAS Key Laboratory of Computational Biology, Chinese Academy of Sciences, Shanghai, China.

Jizhou Yan (J)

Department of Developmental Biology, And Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University, Shanghai, China; CellGene Bioscience, Shanghai, China. Electronic address: jyan2@shou.edu.cn.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Protéines morphogénétiques osseuses › Protéine morphogénétique osseuse de type 4 › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Mécanotransduction cellulaire › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Keratin5-cytoskeleton-BMP4 network regulates...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de poisson › Protéines de poisson-zèbre › Keratin5-cytoskeleton-BMP4 network regulates...