Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 07 2022
07 07 2022
Historique:
received:
20
01
2022
accepted:
16
06
2022
entrez:
7
7
2022
pubmed:
8
7
2022
medline:
12
7
2022
Statut:
epublish
Résumé
Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.
Identifiants
pubmed: 35798722
doi: 10.1038/s41467-022-31414-0
pii: 10.1038/s41467-022-31414-0
pmc: PMC9262879
doi:
Substances chimiques
DNA Polymerase III
EC 2.7.7.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3933Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2022. The Author(s).
Références
BMC Bioinformatics. 2009 Jul 27;10:232
pubmed: 19635165
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
New Phytol. 2017 Mar;213(4):1632-1641
pubmed: 28116755
Mol Plant Pathol. 2016 Sep;17(7):1095-110
pubmed: 26662210
Theor Appl Genet. 2015 Mar;128(3):501-16
pubmed: 25548806
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Front Plant Sci. 2020 Jul 15;11:1069
pubmed: 32760417
BMC Plant Biol. 2018 Jun 25;18(1):132
pubmed: 29940871
BMC Plant Biol. 2009 Sep 11;9:118
pubmed: 19747391
Theor Appl Genet. 2002 Sep;105(4):521-525
pubmed: 12582500
Crop Prot. 2019 Jan;115:104-112
pubmed: 30739973
Gigascience. 2022 Mar 24;11:
pubmed: 35333302
Nat Struct Mol Biol. 2009 Sep;16(9):979-86
pubmed: 19718023
Nature. 2020 Jul;583(7814):90-95
pubmed: 32499645
Virol J. 2017 Mar 7;14(1):47
pubmed: 28270156
BMC Bioinformatics. 2015 Mar 06;16:73
pubmed: 25887893
Front Plant Sci. 2014 Jun 27;5:307
pubmed: 25018765
Front Plant Sci. 2021 Jun 21;12:651992
pubmed: 34234794
Nat Commun. 2021 May 13;12(1):2780
pubmed: 33986276
Nat Biotechnol. 2016 May;34(5):562-70
pubmed: 27088722
Front Plant Sci. 2017 Jan 12;7:2060
pubmed: 28127301
Curr Opin Plant Biol. 2017 Aug;38:50-58
pubmed: 28477536
Plant Mol Biol. 2022 Jun;109(3):195-213
pubmed: 32734418
Plant Biotechnol J. 2021 Apr;19(4):844-854
pubmed: 33190345
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Mol Model. 2019 Feb 27;25(3):80
pubmed: 30810803
Bioinformatics. 2019 Jun 1;35(12):2084-2092
pubmed: 30395178
Plant J. 2013 Apr;74(1):174-83
pubmed: 23289725
Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):157-62
pubmed: 19966286
Science. 2017 Mar 3;355(6328):962-965
pubmed: 28154240
Mol Plant Pathol. 2004 Mar 1;5(2):149-56
pubmed: 20565592
PLoS Genet. 2015 Feb 18;11(2):e1004975
pubmed: 25693187
Plant Methods. 2018 Aug 27;14:73
pubmed: 30154909
Gigascience. 2021 Feb 16;10(2):
pubmed: 33590861
Plant Mol Biol. 2022 Jun;109(3):301-311
pubmed: 34240309
Nat Rev Microbiol. 2013 Nov;11(11):777-88
pubmed: 24100361
Protein Sci. 2021 Jan;30(1):70-82
pubmed: 32881101
Epigenetics Chromatin. 2020 Oct 7;13(1):42
pubmed: 33028374
Plant Genome. 2016 Jul;9(2):
pubmed: 27898832
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
Bioinformatics. 2011 Aug 1;27(15):2156-8
pubmed: 21653522
Virus Res. 2014 Jun 24;186:87-96
pubmed: 24389096
Plant Genome. 2018 Jul;11(2):
pubmed: 30025013
Fly (Austin). 2012 Apr-Jun;6(2):80-92
pubmed: 22728672
BMC Biol. 2019 Sep 18;17(1):75
pubmed: 31533702
Sci Rep. 2019 Dec 18;9(1):19365
pubmed: 31852928
Curr Protoc Bioinformatics. 2013;43:11.10.1-11.10.33
pubmed: 25431634
Nature. 2015 Sep 24;525(7570):533-7
pubmed: 26352475
Plant J. 2021 Dec;108(6):1830-1848
pubmed: 34661327
Trends Genet. 2021 May;37(5):476-487
pubmed: 33608117
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Nucleic Acids Res. 2015 Jan;43(Database issue):D1036-41
pubmed: 25428362
Nat Genet. 2017 Jun;49(6):959-963
pubmed: 28416819
Nat Methods. 2017 Apr;14(4):417-419
pubmed: 28263959