Low protein expression enhances phenotypic evolvability by intensifying selection on folding stability.
Journal
Nature ecology & evolution
ISSN: 2397-334X
Titre abrégé: Nat Ecol Evol
Pays: England
ID NLM: 101698577
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
17
12
2021
accepted:
19
05
2022
pubmed:
8
7
2022
medline:
6
8
2022
entrez:
7
7
2022
Statut:
ppublish
Résumé
Protein abundance affects the evolution of protein genotypes, but we do not know how it affects the evolution of protein phenotypes. Here we investigate the role of protein abundance in the evolvability of green fluorescent protein (GFP) towards the novel phenotype of cyan fluorescence. We evolve GFP in E. coli through multiple cycles of mutation and selection and show that low GFP expression facilitates the evolution of cyan fluorescence. A computational model whose predictions we test experimentally helps explain why: lowly expressed proteins are under stronger selection for proper folding, which facilitates their evolvability on short evolutionary time scales. The reason is that high fluorescence can be achieved by either few proteins that fold well or by many proteins that fold less well. In other words, we observe a synergy between a protein's scarcity and its stability. Because many proteins meet the essential requirements for this scarcity-stability synergy, it may be a widespread mechanism by which low expression helps proteins evolve new phenotypes and functions.
Identifiants
pubmed: 35798838
doi: 10.1038/s41559-022-01797-w
pii: 10.1038/s41559-022-01797-w
pmc: PMC7613228
mid: EMS145180
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1155-1164Subventions
Organisme : Swiss National Science Foundation
ID : 172887
Pays : Switzerland
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Limited.
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