Construction of Streptococcus agalactiae sialic acid mutant and evaluation of its potential as a live attenuated vaccine in Nile tilapia (Oreochromis niloticus).
Oreochromis niloticus
Streptococcus agalactiae
immune protection
live attenuated vaccine
sialic acid
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
07
06
2022
received:
08
11
2021
accepted:
04
07
2022
pubmed:
9
7
2022
medline:
28
9
2022
entrez:
8
7
2022
Statut:
ppublish
Résumé
This study aimed to develop a live attenuated vaccine as an effective approach to prevent streptococcosis in tilapia (Oreochromis niloticus). We eliminated the virulence factor, sialic acid (Sia) encoded by the neuA-D gene cluster from the Group B Streptococcus (Streptococcus agalactiae, GBS) strain WC1535, to construct Sia-deficient S. agalactiae (ΔSia) mutant by homologous recombination. Results showed that the ΔSia mutant had higher adherence to HEp-2 cells and lower resistance to RAW264.7 cell phagocytosis than the wild-type S. agalactiae. The virulence of the ΔSia mutant to tilapia dramatically decreased with no virulence recovery. The relative percent survivals (RPSs) were 50.00% and 54.50% at 30 days when challenged at the wild-type WC1535 doses of 1.0 × 10 GBS Sia plays a critical role in adherence to HEp-2 cells and resistance to the immune clearance of RAW264.7 cells. Moreover, the ΔSia mutant is a safe, stable, and immunogenic live attenuated vaccine candidate to protect tilapia against GBS infection. The results offer more evidence of the importance of Sia in GBS and may be instructive in the control of tilapia streptococcosis.
Substances chimiques
Tumor Necrosis Factor-alpha
0
Vaccines, Attenuated
0
Virulence Factors
0
N-Acetylneuraminic Acid
GZP2782OP0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2403-2416Subventions
Organisme : "key technology and development of food safety" project of MOST (2019YFC1606000)
Organisme : Key Project of Scientific & Technological Innovation of Hubei Province (2018ABA101)
Organisme : the National Key Research and Development Program of China (Grant No. 2020YFD0900300)
Organisme : Wuhan Science and Technology Project (2019020701011480)
Informations de copyright
© 2022 Society for Applied Microbiology.
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