Functional characterization of dopamine and norepinephrine transport across the apical and basal plasma membranes of the human placental syncytiotrophoblast.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 07 2022
08 07 2022
Historique:
received:
27
04
2022
accepted:
29
06
2022
entrez:
8
7
2022
pubmed:
9
7
2022
medline:
14
7
2022
Statut:
epublish
Résumé
The human placenta represents a unique non-neuronal site of monoamine transporter expression, with pathophysiological relevance during the prenatal period. Monoamines (serotonin, dopamine, norepinephrine) are crucial neuromodulators for proper placenta functions and fetal development, including cell proliferation, differentiation, and neuronal migration. Accumulating evidence suggests that even a transient disruption of monoamine balance during gestation may lead to permanent changes in the fetal brain structures and functions, projecting into adulthood. Nonetheless, little is known about the transfer of dopamine and norepinephrine across the placental syncytiotrophoblast. Employing the method of isolated membranes from the human term placenta, here we delineate the transport mechanisms involved in dopamine and norepinephrine passage across the apical microvillous (MVM) and basal membranes. We show that the placental uptake of dopamine and norepinephrine across the mother-facing MVM is mediated via the high-affinity and low-capacity serotonin (SERT/SLC6A4) and norepinephrine (NET/SLC6A2) transporters. In the fetus-facing basal membrane, however, the placental uptake of both monoamines is controlled by the organic cation transporter 3 (OCT3/SLC22A3). Our findings thus provide insights into physiological aspects of dopamine and norepinephrine transport across both the maternal and fetal sides of the placenta. As monoamine transporters represent targets for several neuroactive drugs such as antidepressants, our findings are pharmacologically relevant to ensure the safety of drug use during pregnancy.
Identifiants
pubmed: 35804076
doi: 10.1038/s41598-022-15790-7
pii: 10.1038/s41598-022-15790-7
pmc: PMC9270497
doi:
Substances chimiques
SLC6A4 protein, human
0
Serotonin Plasma Membrane Transport Proteins
0
Serotonin
333DO1RDJY
Dopamine
VTD58H1Z2X
Norepinephrine
X4W3ENH1CV
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11603Informations de copyright
© 2022. The Author(s).
Références
Acta Physiol (Oxf). 2020 Aug;229(4):e13478
pubmed: 32311818
Pharmacol Rev. 2020 Jan;72(1):253-319
pubmed: 31852803
Am J Epidemiol. 2009 Oct 15;170(8):1014-24
pubmed: 19741043
Placenta. 1996 May;17(4):201-7
pubmed: 8761963
Life Sci. 1998;62(15):1323-32
pubmed: 9566774
Med Hypotheses. 2007;68(1):46-60
pubmed: 16959433
Dev Neurosci. 2004 Mar-Aug;26(2-4):229-44
pubmed: 15711063
Eur J Obstet Gynecol Reprod Biol. 1996 Nov;69(2):61-7
pubmed: 8902434
Nat Commun. 2019 Apr 11;10(1):1687
pubmed: 30976000
Neurosci Lett. 2001 Aug 31;309(3):173-6
pubmed: 11514069
Nat Commun. 2021 Apr 13;12(1):2199
pubmed: 33850134
Am J Obstet Gynecol. 1984 Feb 15;148(4):474-81
pubmed: 6696006
Biol Neonate. 1997;71(2):102-10
pubmed: 9057993
J Neurosci. 2002 Jan 15;22(2):389-95
pubmed: 11784783
J Clin Endocrinol Metab. 1978 Jul;47(1):168-70
pubmed: 45461
Clin Chem. 1993 Aug;39(8):1675-8
pubmed: 8353954
Am J Physiol. 1992 May;262(5 Pt 1):C1189-96
pubmed: 1590360
Nature. 1995 Apr 13;374(6523):643-6
pubmed: 7715704
J Neurochem. 2006 Apr;97(2):310-33
pubmed: 16539676
Pharmaceutics. 2021 Aug 20;13(8):
pubmed: 34452265
Methods Mol Med. 2006;122:253-64
pubmed: 16511986
J Pharmacol Exp Ther. 1998 Feb;284(2):736-43
pubmed: 9454822
Am J Med Genet. 2001 Apr 15;100(1):30-6
pubmed: 11337745
J Neurosci Res. 2021 Jan;99(1):271-283
pubmed: 32108381
Brain Res. 2007 Nov 28;1182:11-25
pubmed: 17950709
Biol Psychiatry. 1992 Apr 15;31(8):794-807
pubmed: 1643194
Nature. 2016 Apr 21;532(7599):334-9
pubmed: 27049939
J Endocrinol. 2000 Sep;166(3):689-95
pubmed: 10974662
Brain Res. 1990 May 7;515(1-2):173-80
pubmed: 2357555
Arch Gynecol Obstet. 2015 Jun;291(6):1271-6
pubmed: 25502185
Int J Rad Appl Instrum B. 1989;16(8):771-4
pubmed: 2533589
J Recept Res. 1990;10(3-4):205-15
pubmed: 2148190
Synapse. 1993 May;14(1):34-9
pubmed: 8511716
Biochim Biophys Acta. 1988 Nov 22;945(2):127-34
pubmed: 2847789
J Membr Biol. 1993 Mar;132(2):147-55
pubmed: 8496946
J Biol Chem. 2014 Sep 12;289(37):25764-73
pubmed: 25063810
FEBS Open Bio. 2017 Jan 09;7(2):237-248
pubmed: 28174689
J Neurosci. 2004 Sep 8;24(36):7888-94
pubmed: 15356201
J Neurosci. 2011 Apr 27;31(17):6605-15
pubmed: 21525301
Trends Neurosci. 1997 Jun;20(6):269-74
pubmed: 9185309
Elife. 2021 Jun 01;10:
pubmed: 34061030
Biochemistry. 1993 Feb 9;32(5):1346-53
pubmed: 8448143
Am J Physiol. 1995 Jul;269(1 Pt 1):E145-9
pubmed: 7631769
Toxicol Appl Pharmacol. 1990 Aug;105(1):66-77
pubmed: 1975464
Am J Obstet Gynecol. 1972 Apr 15;112(8):1068-75
pubmed: 5017637
Placenta. 2004 Jul;25(6):518-29
pubmed: 15135235
J Biol Chem. 1979 May 25;254(10):3833-9
pubmed: 438162
J Neurosci. 2013 Jun 19;33(25):10534-43
pubmed: 23785165
Am J Obstet Gynecol. 1982 Sep 15;144(2):149-53
pubmed: 7114122
Neurochem Int. 2019 Feb;123:46-49
pubmed: 30055194
Eur J Pharmacol. 1990 Feb 20;177(1-2):91-4
pubmed: 1967129
Neurochem Int. 2020 May;135:104706
pubmed: 32092327
Baillieres Clin Endocrinol Metab. 1989 Nov;3(3):689-705
pubmed: 2698151
J Neurosci. 2003 Apr 1;23(7):2840-50
pubmed: 12684471
Int J Biochem Cell Biol. 2018 Dec;105:35-40
pubmed: 30266525
Drugs Future. 2004 Dec;29(12):1235-1244
pubmed: 16871320