Volatilomic Signatures of AGS and SNU-1 Gastric Cancer Cell Lines.


Journal

Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009

Informations de publication

Date de publication:
22 Jun 2022
Historique:
received: 17 05 2022
revised: 18 06 2022
accepted: 18 06 2022
entrez: 9 7 2022
pubmed: 10 7 2022
medline: 14 7 2022
Statut: epublish

Résumé

In vitro studies can help reveal the biochemical pathways underlying the origin of volatile indicators of numerous diseases. The key objective of this study is to identify the potential biomarkers of gastric cancer. For this purpose, the volatilomic signatures of two human gastric cancer cell lines, AGS (human gastric adenocarcinoma) and SNU-1 (human gastric carcinoma), and one normal gastric mucosa cell line (GES-1) were investigated. More specifically, gas chromatography mass spectrometry has been applied to pinpoint changes in cell metabolism triggered by cancer. In total, ten volatiles were found to be metabolized, and thirty-five were produced by cells under study. The volatiles consumed were mainly six aldehydes and two heterocyclics, whereas the volatiles released embraced twelve ketones, eight alcohols, six hydrocarbons, three esters, three ethers, and three aromatic compounds. The SNU-1 cell line was found to have significantly altered metabolism in comparison to normal GES-1 cells. This was manifested by the decreased production of alcohols and ketones and the upregulated emission of esters. The AGS cells exhibited the increased production of methyl ketones containing an odd number of carbons, namely 2-tridecanone, 2-pentadecanone, and 2-heptadecanone. This study provides evidence that the cancer state modifies the volatilome of human cells.

Identifiants

pubmed: 35807254
pii: molecules27134012
doi: 10.3390/molecules27134012
pmc: PMC9268292
pii:
doi:

Substances chimiques

Alcohols 0
Esters 0
Ketones 0
Volatile Organic Compounds 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : European Union
ID : 824986
Organisme : National Science Centre, Poland
ID : 2021/41/N/ST4/02307

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Auteurs

Daria Ślefarska-Wolak (D)

Institute for Breath Research, University of Innsbruck, 6850 Dornbirn, Austria.
Institute of Chemistry, Jan Kochanowski University, 25406 Kielce, Poland.

Christine Heinzle (C)

Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria.
Medical Central Laboratories, 6800 Feldkirch, Austria.

Andreas Leiherer (A)

Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria.
Medical Central Laboratories, 6800 Feldkirch, Austria.
Private University in the Principality of Liechtenstein, 9495 Triesen, Liechtenstein.

Clemens Ager (C)

Institute for Breath Research, University of Innsbruck, 6850 Dornbirn, Austria.

Axel Muendlein (A)

Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria.

Linda Mezmale (L)

Institute of Clinical and Preventive Medicine, Faculty of Medicine, University of Latvia, 1586 Riga, Latvia.
Riga East University Hospital, 1586 Riga, Latvia.

Marcis Leja (M)

Institute of Clinical and Preventive Medicine, Faculty of Medicine, University of Latvia, 1586 Riga, Latvia.
Riga East University Hospital, 1586 Riga, Latvia.
Digestive Diseases Centre GASTRO, 1586 Riga, Latvia.

Alejandro H Corvalan (AH)

Advanced Center for Chronic Diseases (ACCDiS), Pontificia Universidad Catolica de Chile, Santiago 833003, Chile.

Heinz Drexel (H)

Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria.
Department of Medicine and Cardiology, Academic Teaching Hospital Bregenz, 6900 Bregenz, Austria.
College of Medicine, Drexel University, Philadelphia, PA 19129, USA.

Agnieszka Królicka (A)

Department of Building Materials Technology, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30059 Krakow, Poland.

Gidi Shani (G)

Department of Chemical Engineering, Russel Berrie Nanotechnology Institute, Technicon-Israel Institute of Technology, Haifa 3200003, Israel.

Christopher A Mayhew (CA)

Institute for Breath Research, University of Innsbruck, 6850 Dornbirn, Austria.
Tiroler Krebsforschungsinstitut (TKFI), 6020 Innsbruck, Austria.

Hossam Haick (H)

Department of Chemical Engineering, Russel Berrie Nanotechnology Institute, Technicon-Israel Institute of Technology, Haifa 3200003, Israel.

Paweł Mochalski (P)

Institute for Breath Research, University of Innsbruck, 6850 Dornbirn, Austria.
Institute of Chemistry, Jan Kochanowski University, 25406 Kielce, Poland.

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Classifications MeSH