Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells.
CRISPR/Cas9
Monosomic XO female mice
Mouse embryonic stem cells
Tetraploid complementation
Y chromosome deletion
Journal
Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
10
7
2022
pubmed:
11
7
2022
medline:
14
7
2022
Statut:
ppublish
Résumé
Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice are produced through spontaneous Y chromosome loss. Here, we describe an efficient method to obtain monosomic XO ES cells by CRISPR/Cas9-mediated deletion of the Y chromosome allowing generation of female clonal mice by tetraploid complementation. The monosomic XO female mice are viable and able to produce normal male and female offspring. Direct generation of clonal mice in both sexes can significantly accelerate the production of complex genetically modified mouse models.
Identifiants
pubmed: 35811100
pii: S0091-679X(22)00032-2
doi: 10.1016/bs.mcb.2022.02.015
pmc: PMC9280658
mid: NIHMS1797502
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
203-210Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM129380
Pays : United States
Organisme : NIH HHS
ID : R21 OD031973
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Disclosures The authors declare no competing interests. D. W declares this work was funded by grants 1 R01 GM129380–01 from the National Institutes of Health and New York State Stem Cell Science Program (NYSTEM Contract C32581GG).
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