Follow that cell: Leukocyte migration in L-plastin mutant zebrafish.
actin
cytoskeleton
immunity
macrophage
neutrophil
zebrafish
Journal
Cytoskeleton (Hoboken, N.J.)
ISSN: 1949-3592
Titre abrégé: Cytoskeleton (Hoboken)
Pays: United States
ID NLM: 101523844
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
revised:
21
06
2022
received:
22
02
2022
accepted:
07
07
2022
pubmed:
12
7
2022
medline:
7
10
2022
entrez:
11
7
2022
Statut:
ppublish
Résumé
Actin assemblies are important in motile cells such as leukocytes, which form dynamic plasma membrane extensions or podia. L-plastin (LCP1) is a leukocyte-specific calcium-dependent actin-bundling protein that, in mammals, is known to affect immune cell migration. Previously, we generated CRISPR/Cas9 engineered zebrafish lacking L-plastin (lcp1-/-) and reported that they had reduced survival to adulthood, suggesting that lack of this actin-bundler might negatively affect the immune system. To test this hypothesis, we examined the distribution and migration of neutrophils and macrophages in the transparent tail of early zebrafish larvae using cell-specific markers and an established wound-migration assay. Knockout larvae were similar to their heterozygous siblings in having equal body sizes and comparable numbers of neutrophils in caudal hematopoietic tissue at 2 days postfertilization, indicating no gross defect in neutrophil production or developmental migration. When stimulated by a tail wound, all genotypes of neutrophils were equally migratory in a two-hour window. However, for macrophages we observed both migration defects and morphological differences. L-plastin knockout macrophages (lcp1 -/-) still homed to wounds but were slower, less directional and had a star-like morphology with many leading and trailing projections. In contrast, heterozygous macrophages lcp1 (+/-) were faster, more directional, and had a streamlined, slug-like morphology. Overall, these findings show that in larval zebrafish L-plastin knockout primarily affects the macrophage response with possible consequences for organismal immunity. Consistent with our observations, we propose a model in which cytoplasmic L-plastin negatively regulates macrophage integrin adhesion by holding these transmembrane heterodimers in a "clasped," inactive form and is a necessary part of establishing macrophage polarity during chemokine-induced motility.
Substances chimiques
Actins
0
Chemokines
0
Integrins
0
Membrane Glycoproteins
0
Microfilament Proteins
0
plastin
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
26-37Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103545
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM098151
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM120664
Pays : United States
Informations de copyright
© 2022 Wiley Periodicals LLC.
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