A label-free SPR biosensor for specific detection of TLR4 expression; introducing of 10-HDA as an antagonist.
Antagonist of TLR4 (10-hydroxy-2-decanoic acid)
HEK293T cells expressingTLR4
Surface plasmon resonance (SPR) biosensor
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
30 Sep 2022
30 Sep 2022
Historique:
received:
24
04
2022
revised:
15
06
2022
accepted:
05
07
2022
pubmed:
12
7
2022
medline:
2
9
2022
entrez:
11
7
2022
Statut:
ppublish
Résumé
Toll-like receptor 4 (TLR4) is actively involved in many health-related problems, including transplantation rejection and autoimmune diseases. Therefore, it is important to identify an antagonist to inhibit the TLR4-induced immune cell activation. In our previous study, 10-hydroxy-2-decanoic acid (10-HDA) was introduced as a potential antagonist for TLR4; however, possible interaction between 10-HDA and TLR4 needed to be detected. Due to the ability of surface plasmon resonance (SPR) biosensor to confirm the specific interactions between receptors and ligands, a new configuration of SPR biosensor proposed to detect the possible interaction between 10-HDA and TLR4. Hence, 10-HDA was immobilized using the (3-aminopropyl) triethoxysilane (APTES) polymer as a crosslinking agent on the Ag-MgF
Identifiants
pubmed: 35817233
pii: S0141-8130(22)01459-3
doi: 10.1016/j.ijbiomac.2022.07.035
pii:
doi:
Substances chimiques
Fatty Acids, Monounsaturated
0
Polymers
0
TLR4 protein, human
0
Toll-Like Receptor 4
0
10-hydroxy-2-decenoic acid
765-01-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
142-149Informations de copyright
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