Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes.
Extracellular vesicles
Fluorescence resonance energy transfer
Liposomes
Membrane fusion
Polyethylene glycol
Ultracentrifugation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
12
7
2022
pubmed:
13
7
2022
medline:
15
7
2022
Statut:
ppublish
Résumé
Extracellular vesicles (EVs) and liposomes are natural and synthetic drug delivery systems, respectively, with their own advantages and limitations. EV/liposome fusion allows the generation of hybrid EVs that benefit from both the versatility of liposomes (tunable lipid and protein composition, surface functionalization, lumen loading, etc.) and the functionality of EVs (natural targeting properties, low immunogenicity, anti-inflammatory properties, etc.). Here, we describe the methods to (1) produce EVs and liposomes, (2) induce and monitor their fusion, and (3) purify the obtained hybrid EVs.
Identifiants
pubmed: 35819777
doi: 10.1007/978-1-0716-2209-4_24
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Liposomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
385-396Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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