TSG-6 released from adipose stem cells-derived small extracellular vesicle protects against spinal cord ischemia reperfusion injury by inhibiting endoplasmic reticulum stress.

Endoplasmic Reticulum Stress Extracellular Vesicles / metabolism Humans Mesenchymal Stem Cells / metabolism Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt Reperfusion Injury / metabolism Spinal Cord / pathology Spinal Cord Ischemia / complications
Adipose‐derived stem cells Endoplasmic reticulum stress Small extracellular vesicle Spinal cord ischemia reperfusion injury Tumor necrosis factor (TNF)-stimulated gene-6

Journal

Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581

Informations de publication

Date de publication:
13 07 2022
Historique:
received: 02 09 2021
accepted: 12 06 2022
entrez: 13 7 2022
pubmed: 14 7 2022
medline: 16 7 2022
Statut: epublish

Résumé

Spinal cord ischemia reperfusion injury (SCIRI) is a complication of aortic aneurysm repair or spinal cord surgery that is associated with permanent neurological deficits. Mesenchymal stem cell (MSC)-derived small extracellular vesicles (sEVs) have been shown to be potential therapeutic options for improving motor functions after SCIRI. Due to their easy access and multi-directional differentiation potential, adipose-derived stem cells (ADSCs) are preferable for this application. However, the effects of ADSC-derived sEVs (ADSC-sEVs) on SCIRI have not been reported. We found that ADSC-sEVs inhibited SCIRI-induced neuronal apoptosis, degradation of tight junction proteins and suppressed endoplasmic reticulum (ER) stress. However, in the presence of the ER stress inducer, tunicamycin, its anti-apoptotic and blood-spinal cord barrier (BSCB) protective effects were significantly reversed. We found that ADSC-sEVs contain tumor necrosis factor (TNF)-stimulated gene-6 (TSG-6) whose overexpression inhibited ER stress in vivo by modulating the PI3K/AKT pathway. ADSC-sEVs inhibit neuronal apoptosis and BSCB disruption in SCIRI by transmitting TSG-6, which suppresses ER stress by modulating the PI3K/AKT pathway.

Sections du résumé

BACKGROUND
Spinal cord ischemia reperfusion injury (SCIRI) is a complication of aortic aneurysm repair or spinal cord surgery that is associated with permanent neurological deficits. Mesenchymal stem cell (MSC)-derived small extracellular vesicles (sEVs) have been shown to be potential therapeutic options for improving motor functions after SCIRI. Due to their easy access and multi-directional differentiation potential, adipose-derived stem cells (ADSCs) are preferable for this application. However, the effects of ADSC-derived sEVs (ADSC-sEVs) on SCIRI have not been reported.
RESULTS
We found that ADSC-sEVs inhibited SCIRI-induced neuronal apoptosis, degradation of tight junction proteins and suppressed endoplasmic reticulum (ER) stress. However, in the presence of the ER stress inducer, tunicamycin, its anti-apoptotic and blood-spinal cord barrier (BSCB) protective effects were significantly reversed. We found that ADSC-sEVs contain tumor necrosis factor (TNF)-stimulated gene-6 (TSG-6) whose overexpression inhibited ER stress in vivo by modulating the PI3K/AKT pathway.
CONCLUSIONS
ADSC-sEVs inhibit neuronal apoptosis and BSCB disruption in SCIRI by transmitting TSG-6, which suppresses ER stress by modulating the PI3K/AKT pathway.

Identifiants

DOI: 10.1186/s13287-022-02963-4 PMID: 35831906 PMC: PMC9281104
pubmed: 35831906
doi: 10.1186/s13287-022-02963-4
pii: 10.1186/s13287-022-02963-4
pmc: PMC9281104
doi:

Substances chimiques

Proto-Oncogene Proteins c-akt EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

291

Informations de copyright

© 2022. The Author(s).

Références

Stem Cells. 2017 Apr;35(4):851-858
pubmed: 28294454
FASEB J. 2019 Sep;33(9):10152-10164
pubmed: 31184927
Theranostics. 2019 May 31;9(14):4084-4100
pubmed: 31281533
Eur J Neurosci. 2016 Aug;44(4):2114-9
pubmed: 27177616
J Nanobiotechnology. 2020 May 13;18(1):72
pubmed: 32404105
EBioMedicine. 2017 Feb;15:137-149
pubmed: 28024734
Prog Neurobiol. 2017 Oct;157:49-78
pubmed: 28322920
Int J Biol Sci. 2019 Jan 1;15(2):277-286
pubmed: 30745820
Cell Death Dis. 2019 Aug 8;10(8):597
pubmed: 31395857
Cell Death Dis. 2020 Jun 1;11(6):409
pubmed: 32483121
Stem Cell Res Ther. 2018 Apr 6;9(1):91
pubmed: 29625582
Neural Regen Res. 2014 Sep 15;9(18):1665-71
pubmed: 25374587
Stem Cell Res Ther. 2021 Aug 3;12(1):433
pubmed: 34344458
J Spinal Disord Tech. 2009 Dec;22(8):565-70
pubmed: 19956030
Exp Neurol. 2018 Sep;307:99-108
pubmed: 29883579
J Neurotrauma. 2019 Feb 1;36(3):469-484
pubmed: 29848167
Neurochem Int. 2014 Mar;68:18-27
pubmed: 24560721
Eur Rev Med Pharmacol Sci. 2019 Jan;23(1):52-60
pubmed: 30657546
Stem Cells. 2017 Jun;35(6):1636-1646
pubmed: 28376564
Semin Cell Dev Biol. 2015 Apr;40:82-8
pubmed: 25765629
Cell Death Dis. 2019 Apr 18;10(5):340
pubmed: 31000697
Brain. 2014 Jul;137(Pt 7):1894-906
pubmed: 24898351
Cell Stem Cell. 2016 Feb 4;18(2):174-88
pubmed: 26849304
Cell. 2010 Mar 19;140(6):900-17
pubmed: 20303879
J Extracell Vesicles. 2019 Apr 29;8(1):1609206
pubmed: 31069028
J Extracell Vesicles. 2020 Oct;10(1):e12024
pubmed: 33304476
Biomaterials. 2017 Sep;140:189-200
pubmed: 28658635
Nature. 2004 Apr 8;428(6983):668-73
pubmed: 15034594
Cell Transplant. 2019 Jul;28(7):874-884
pubmed: 31185737
J Neurotrauma. 2006 Nov;23(11):1654-70
pubmed: 17115911
Aging (Albany NY). 2019 Sep 28;11(18):7723-7745
pubmed: 31563124
Nature. 2003 Dec 18;426(6968):891-4
pubmed: 14685249
Cell Metab. 2012 May 2;15(5):623-34
pubmed: 22560215
J Vasc Surg. 2010 Dec;52(6):1580-6
pubmed: 20843623
Brain. 2020 May 1;143(5):1383-1399
pubmed: 32419025
Matrix Biol. 2019 May;78-79:60-83
pubmed: 29362135
Stem Cell Res Ther. 2021 May 29;12(1):315
pubmed: 34051868
Neurobiol Dis. 2020 Nov;145:105077
pubmed: 32898645
Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7586-91
pubmed: 20368433
Ann Transl Med. 2017 Feb;5(4):83
pubmed: 28275628
J Neuroinflammation. 2020 Apr 6;17(1):101
pubmed: 32248810
J Nanobiotechnology. 2020 Jul 25;18(1):105
pubmed: 32711535
Cell Stem Cell. 2015 Jul 2;17(1):11-22
pubmed: 26140604
Int J Biol Sci. 2017 Nov 27;13(12):1520-1531
pubmed: 29230100
J Neurotrauma. 2006 May;23(5):635-59
pubmed: 16689667
J Biol Chem. 2014 Mar 28;289(13):9430-9
pubmed: 24509861
Mol Ther. 2015 May;23(5):812-823
pubmed: 25868399
Stem Cell Res Ther. 2018 Sep 26;9(1):255
pubmed: 30257717
Brain Res. 2014 May 2;1561:1-10
pubmed: 24613403
Ann Cardiothorac Surg. 2012 Sep;1(3):286-92
pubmed: 23977510
Oxid Med Cell Longev. 2017;2017:8640284
pubmed: 28685010
Am J Transl Res. 2019 Feb 15;11(2):780-792
pubmed: 30899379
Stem Cell Res. 2019 Dec;41:101601
pubmed: 31731179
J Neuroinflammation. 2018 May 18;15(1):150
pubmed: 29776446
J Neuroinflammation. 2018 Aug 20;15(1):231
pubmed: 30126439
Circ Res. 2011 May 27;108(11):1340-7
pubmed: 21493893
Cell Death Dis. 2018 Feb 12;9(2):212
pubmed: 29434185
Brain. 2012 Aug;135(Pt 8):2375-89
pubmed: 22798270
J Neurotrauma. 2018 Jun 15;35(12):1329-1344
pubmed: 29316847
Osteoarthritis Cartilage. 2018 Feb;26(2):245-254
pubmed: 29129649

Auteurs

Xiao Lu (X)

Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.
Department of Orthopaedics, Dongtai Hospital Affiliated to Nantong University, Dongtai City, Jiangsu, China.

Chengtang Lv (C)

Department of Orthopaedics, Yancheng Third People's Hospital, Yancheng, 224000, Jiangsu, China.

Yuechao Zhao (Y)

Department of Orthopedic Oncology, Changzheng Hospital, Secondary Military Medical University, Shanghai, China.
Department of Orthopedic, PLA Navy No.905 Hospital, Secondary Military Medical University, Shanghai, China.

Yufei Wang (Y)

Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Haining, Zhejiang, China.

Yao Li (Y)

Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, China.

Chengyue Ji (C)

Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.

Zhuanghui Wang (Z)

Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.

Wu Ye (W)

Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.

Shunzhi Yu (S)

Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, China. maotoutou@163.com.

Jianling Bai (J)

Department of Biostatistics, School of Public Health, Nanjing Medical University, Jiangsu Province, Nanjing, 211166, China. baijianling@njmu.edu.cn.

Weihua Cai (W)

Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China. caiweihuazhuren@163.com.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Stress du réticulum endoplasmique › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphatidylinositol 3-kinases › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-akt › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Complications postopératoires › Lésion d'ischémie-reperfusion › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Système nerveux › Système nerveux central › Moelle spinale › TSG-6 released from adipose stem cells-derived...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Maladies vasculaires de la moelle épinière › Ischémie de la moelle épinière › TSG-6 released from adipose stem cells-derived...