Characterization of PRRSV in clinical samples and the corresponding cell culture isolates.
MARC-145
ORF5
PRRSV
ZMAC
mixed infection
next-generation sequencing
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
11
07
2022
received:
27
05
2022
accepted:
13
07
2022
pubmed:
16
7
2022
medline:
30
9
2022
entrez:
15
7
2022
Statut:
ppublish
Résumé
Isolation of porcine reproductive and respiratory syndrome virus (PRRSV) in cell culture is a primary means of obtaining virus isolates for autogenous vaccine production and other applications. However, it has not been well characterized whether cell culture isolate and the virus in clinical sample are equivalent. This study compared PRRSV ORF5 sequences from 1024 clinical samples (995 PRRSV-2, 26 PRRSV-1, and three PRRSV-1 and PRRSV-2 PCR-positive) and their isolates in MARC-145 and/or ZMAC cells. For three PRRSV-1 and PRRSV-2 PCR-positive clinical samples, both PRRSV-1 and PRRSV-2 were isolated in ZMAC cells, whereas either PRRSV-1 or PRRSV-2, but not both, was isolated in MARC-145 cells, with isolate sequences matching the respective viruses in clinical samples. Twenty-six PRRSV-1 and most of 995 PRRSV-2 PCR-positive clinical samples had matching viral ORF5 sequences with their cell culture isolates. However, 14 out of 995 PRRSV-2 cases (1.4%) had nonmatching viral sequences between clinical samples and MARC-145 isolates, although viral sequences from clinical samples and ZMAC isolates matched. This is concerning because, if the MARC-145 isolate is directly used for autogenous vaccine production without sequencing confirmation against the virus in the clinical sample, it is possible that the produced autogenous vaccine does not include the desired wild-type virus strain found on the farm and instead contains vaccine-like virus. Vaccine-specific PCR and next-generation sequencing performed on six selected cases indicated presence of ≥2 PRRSV-2 strains (mixed infection) in such clinical samples. In summary, PRRSV ORF5 sequences from clinical samples and cell culture isolates matched each other for majority of the cases. However, PRRSV sequences between clinical sample and MARC-145 cell culture isolate could occasionally be different when the clinical sample contains ≥2 PRRSV-2 strains. Characterizing PRRSV sequences from clinical samples and cell culture isolates should be conducted before using isolates for producing autogenous vaccines or other applications.
Substances chimiques
Autovaccines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3045-e3059Subventions
Organisme : American Association of Swine Veterinarians Foundation
Organisme : Iowa Livestock Health Advisory Council
Organisme : MorriSTONE Faculty Fellowship
Organisme : Iowa State University Veterinary Diagnostic Laboratory
Informations de copyright
© 2022 Wiley-VCH GmbH.
Références
Andino, R., & Domingo, E. (2015). Viral quasispecies. Virology, 479-480, 46-51. https://doi.org/10.1016/j.virol.2015.03.022
Calzada-Nova, G., Husmann, R. J., Schnitzlein, W. M., & Zuckermann, F. A. (2012). Effect of the host cell line on the vaccine efficacy of an attenuated porcine reproductive and respiratory syndrome virus. Veterinary Immunology and Immunopathology, 148(1-2), 116-125. https://doi.org/10.1016/j.vetimm.2012.05.008
Cha, S. H., Chang, C. C., & Yoon, K. J. (2004). Instability of the restriction fragment length polymorphism pattern of open reading frame 5 of porcine reproductive and respiratory syndrome virus during sequential pig-to-pig passages. Journal of Clinical Microbiology, 42(10), 4462-4467. https://doi.org/10.1128/jcm.42.10.4462-4467.2004
Chen, N., Dekkers, J. C. M., Ewen, C. L., & Rowland, R. R. R. (2015). Porcine reproductive and respiratory syndrome virus replication and quasispecies evolution in pigs that lack adaptive immunity. Virus Research, 195, 246-249. https://doi.org/10.1016/j.virusres.2014.10.006
Dea, S., Sawyer, N., Alain, R., & Athanassious, R. (1995). Ultrastructural characteristics and morphogenesis of porcine reproductive and respiratory syndrome virus propagated in the highly permissive MARC-145 cell clone. Advances in Experimental Medicine and Biology, 380, 95-98. https://doi.org/10.1007/978-1-4615-1899-0_13
Domingo, E., Sheldon, J., & Perales, C. (2012). Viral quasispecies evolution. Microbiology and Molecular Biology Reviews, 76(2), 159-216. https://doi.org/10.1128/MMBR.05023-11
Gauger, P., & Harmon, K. (2021). PRRS CLAMP: Molecular diagnostic tools to distinguish wild-type and vaccine strains for PRRSV. Paper presented at the Annual Meeting of American Association of Swine Veterinarians.
Goldberg, T. L., Lowe, J. F., Milburn, S. M., & Firkins, L. D. (2003). Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection. Virology, 317(2), 197-207. https://doi.org/10.1016/j.virol.2003.07.009
Han, J., Wang, Y., & Faaberg, K. S. (2006). Complete genome analysis of RFLP 184 isolates of porcine reproductive and respiratory syndrome virus. Virus Research, 122(1-2), 175-182. https://doi.org/10.1016/j.virusres.2006.06.003
Holtkamp, D., Kliebenstein, J., Neumann, E., Zimmerman, J., Rotto, H., Yoder, T., Wang, C., Yeske, P., Mowrer, C L., & Haley, C. (2013). Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on U.S. pork producers. Journal of Swine Health and Production, 21, 72-84.
Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30(4), 772-780. https://doi.org/10.1093/molbev/mst010
Lu, Z. H., Wang, X., Wilson, A. D., Dorey-Robinson, D. L. W., Archibald, A. L., Ait-Ali, T., & Frossard, J. - P. (2017). Quasispecies evolution of the prototypical genotype 1 porcine reproductive and respiratory syndrome virus early during in vivo infection is rapid and tissue specific. Archives of Virology, 162(8), 2203-2210. https://doi.org/10.1007/s00705-017-3342-0
Lunney, J. K., Fang, Y., Ladinig, A., Chen, N., Li, Y., Rowland, B., & Renukaradhya, G. J. (2016). Porcine reproductive and respiratory syndrome virus (PRRSV): Pathogenesis and interaction with the immune system. Annual Review of Animal Biosciences, 4, 129-154. https://doi.org/10.1146/annurev-animal-022114-111025
Nguyen, L. T., Schmidt, H. A., von Haeseler, A., & Minh, B. Q. (2015). IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, 32(1), 268-274. https://doi.org/10.1093/molbev/msu300
Paploski, I., Pamornchainavakul, N., Makau, D. N., Rovira, A., Corzo, C., Schroeder, D. C., Cheeran, M., Doeschl-Wilson, A., Kao, R. R., Lycett, S., & VanderWaal, K. (2021). Phylogenetic structure and sequential dominance of sub-lineages of PRRSV type-2 lineage 1 in the United States. Vaccines, 9, 608.
Paploski, I. A. D., Corzo, C., Rovira, A., Murtaugh, M. P., Sanhueza, J. M., Vilalta, C., Schroeder, D C., & VanderWaal, K. (2019). Temporal dynamics of co-circulating lineages of porcine reproductive and respiratory syndrome virus. Frontiers in Microbiology, 10, 2486. https://doi.org/10.3389/fmicb.2019.02486
Rawal, G., Yim-Im, W., Chamba, F., Smith, C., Okones, J., Francisco, C., & Zhang, J. (2022). Development and validation of a reverse transcription real-time PCR assay for specific detection of PRRSGard vaccine-like virus. Transboundary and Emerging Diseases, 69(3), 1212-1226. https://doi.org/10.1111/tbed.14084
Renukaradhya, G. J., Meng, X. - J., Calvert, J. G., Roof, M., & Lager, K. M. (2015). Live porcine reproductive and respiratory syndrome virus vaccines: Current status and future direction. Vaccine, 33(33), 4069-4080. https://doi.org/10.1016/j.vaccine.2015.06.092
Robinson, J. T., Thorvaldsdóttir, H., Winckler, W., Guttman, M., Lander, E. S., Getz, G., & Mesirov, J. P. (2011). Integrative genomics viewer. Nature Biotechnology, 29(1), 24-26. https://doi.org/10.1038/nbt.1754
Rowland, R. R., Steffen, M., Ackerman, T., & Benfield, D. A. (1999). The evolution of porcine reproductive and respiratory syndrome virus: Quasispecies and emergence of a virus subpopulation during infection of pigs with VR-2332. Virology, 259(2), 262-266. https://doi.org/10.1006/viro.1999.9789
Shi, M., Lam, T. T., Hon, C. C., Hui, R. K., Faaberg, K. S., Wennblom, T., Murtaugh, M P., Stadejek, T., & Leung, F. C. (2010). Molecular epidemiology of PRRSV: A phylogenetic perspective. Virus Research, 154(1-2), 7-17. https://doi.org/10.1016/j.virusres.2010.08.014
Shi, M., Lam, T. T., Hon, C. C., Murtaugh, M. P., Davies, P. R., Hui, R. K., Li, J., Tik-Wim Wong, L., Yip, C.-W., Jiang, J.-W., & Leung, F. C. (2010). Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. Journal of Virology, 84(17), 8700-8711. https://doi.org/10.1128/JVI.02551-09
Shi, M., Lemey, P., Brar, M. S., Suchard, M. A., Murtaugh, M. P., Carman, S., D'Allaire, S., Delisle, B., Lambert, M.-È., Gagnon, C. A., Ge, L., Qu, Y., Yoo, D., Holmes, E. C., & Chi-Ching Leung, F. (2013). The spread of type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in North America: A phylogeographic approach. Virology, 447(1-2), 146-154. https://doi.org/10.1016/j.virol.2013.08.028
Snijder, E. J., Kikkert, M., & Fang, Y. (2013). Arterivirus molecular biology and pathogenesis. Journal of General Virology, 94(Pt 10), 2141-2163. https://doi.org/10.1099/vir.0.056341-0
Stadejek, T., Oleksiewicz, M. B., Scherbakov, A. V., Timina, A. M., Krabbe, J. S., Chabros, K., & Potapchuk, D. (2008). Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: Nucleocapsid characteristics and geographical distribution in Europe. Archives of Virology, 153(8), 1479-1488. https://doi.org/10.1007/s00705-008-0146-2
Stadejek, T., Stankevicius, A., Murtaugh, M. P., & Oleksiewicz, M. B. (2013). Molecular evolution of PRRSV in Europe: Current state of play. Veterinary Microbiology, 165(1-2), 21-28. https://doi.org/10.1016/j.vetmic.2013.02.029
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197
Trevisan, G., Sharma, A., Gauger, P., Harmon, K. M., Zhang, J., Main, R., Zeller, M., Linhares, L C. M., & Linhares, D. C. L. (2021). PRRSV2 genetic diversity defined by RFLP patterns in the United States from 2007 to 2019. Journal of Veterinary Diagnostic Investigation, 33(5), 920-931. https://doi.org/10.1177/10406387211027221
Trevisan, G., Zeller, M., Li, G., Zhang, J., Gauger, P., & Linhares, D. C. L. (2022). Implementing a user-friendly format to analyze PRRSV next-generation sequencing results and associating breeding herd production performance with number of PRRSV strains and recombination events. Transboundary and Emerging Diseases, 69(5), e2214-e2229. https://doi.org/10.1111/tbed.14560
Wang, Y., Yim-Im, W., Porter, E., Lu, N., Anderson, J., Noll, L., Zhang, J., & Bai, J. (2021). Development of a bead-based assay for detection and differentiation of field strains and four vaccine strains of type 2 porcine reproductive and respiratory syndrome virus (PRRSV-2) in the USA. Transboundary and Emerging Diseases, 68(3), 1414-1423. https://doi.org/10.1111/tbed.13808
Yim-im, W., Huang, H., Park, J., Wang, C., Calzada, G., Gauger, P., Harmon, K., Main, R., & Zhang, J. (2021). Comparison of ZMAC and MARC-145 cell lines for improving porcine reproductive and respiratory syndrome virus isolation from clinical samples. Journal of Clinical Microbiology, 59(3), e01757-01720. https://doi.org/10.1128/JCM.01757-20
Yoon, K. J., Chang, C. C., Zimmerman, J., & Harmon, K. (2001). Genetic and antigenic stability of PRRS virus in pigs. Field and experimental prospectives. Advances in Experimental Medicine and Biology, 494, 25-30.
Zhang, J., Zheng, Y., Xia, X. Q., Chen, Q., Bade, S. A., Yoon, K. J., Harmon, K M., Gauger, P C., Main, R G., & Li, G. (2017). High-throughput whole genome sequencing of Porcine reproductive and respiratory syndrome virus from cell culture materials and clinical specimens using next-generation sequencing technology. Journal of Veterinary Diagnostic Investigation, 29(1), 41-50. https://doi.org/10.1177/1040638716673404
Zhao, P., Ma, C., Dong, X., & Cui, Z. (2012). Evolution of quasispecies diversity for porcine reproductive and respiratory syndrome virus under antibody selective pressure. Science China Life Sciences, 55(9), 788-792. https://doi.org/10.1007/s11427-012-4364-1
Zhou, L., Ge, X., & Yang, H. (2021). Porcine reproductive and respiratory syndrome modified live virus vaccine: A “leaky” vaccine with debatable efficacy and safety. Vaccines, 9(4), 362.
Zimmerman, J., Dee, S., Holtkamp, D. J., Murtaugh, M., Stadejek, T., Stevenson, G., Stevenson, G W., Torremorell, M., Yang, H., &. Zhang, J. (2019). Porcine reproductive and respiratory syndrome viruses (porcine arteriviruses). In J. J. Zimmerman, L. A. Karriker, A. Ramirez, K. J. Schwartz, G. W. Stevenson, & J. Zhang (Eds.), Diseases of swine (11th ed., pp. 685-708). Wiley-Blackwell.