Caspase-1-driven neutrophil pyroptosis and its role in host susceptibility to Pseudomonas aeruginosa.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
27
01
2022
accepted:
01
06
2022
revised:
02
08
2022
pubmed:
19
7
2022
medline:
5
8
2022
entrez:
18
7
2022
Statut:
epublish
Résumé
Multiple regulated neutrophil cell death programs contribute to host defense against infections. However, despite expressing all necessary inflammasome components, neutrophils are thought to be generally defective in Caspase-1-dependent pyroptosis. By screening different bacterial species, we found that several Pseudomonas aeruginosa (P. aeruginosa) strains trigger Caspase-1-dependent pyroptosis in human and murine neutrophils. Notably, deletion of Exotoxins U or S in P. aeruginosa enhanced neutrophil death to Caspase-1-dependent pyroptosis, suggesting that these exotoxins interfere with this pathway. Mechanistically, P. aeruginosa Flagellin activates the NLRC4 inflammasome, which supports Caspase-1-driven interleukin (IL)-1β secretion and Gasdermin D (GSDMD)-dependent neutrophil pyroptosis. Furthermore, P. aeruginosa-induced GSDMD activation triggers Calcium-dependent and Peptidyl Arginine Deaminase-4-driven histone citrullination and translocation of neutrophil DNA into the cell cytosol without inducing extracellular Neutrophil Extracellular Traps. Finally, we show that neutrophil Caspase-1 contributes to IL-1β production and susceptibility to pyroptosis-inducing P. aeruginosa strains in vivo. Overall, we demonstrate that neutrophils are not universally resistant for Caspase-1-dependent pyroptosis.
Identifiants
pubmed: 35849616
doi: 10.1371/journal.ppat.1010305
pii: PPATHOGENS-D-22-00169
pmc: PMC9345480
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
Exotoxins
0
Inflammasomes
0
Interleukin-1beta
0
Caspase 1
EC 3.4.22.36
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010305Subventions
Organisme : NIAMS NIH HHS
ID : P30 AR073752
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cell Rep. 2020 Jul 28;32(4):107967
pubmed: 32726630
J Am Soc Nephrol. 2015 Oct;26(10):2399-413
pubmed: 25644111
PLoS Pathog. 2021 Sep 13;17(9):e1009927
pubmed: 34516571
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15880-5
pubmed: 20798043
Science. 2018 Nov 23;362(6417):956-960
pubmed: 30467171
EMBO Rep. 2020 Nov 5;21(11):e50829
pubmed: 33124769
J Exp Med. 2010 Aug 30;207(9):1853-62
pubmed: 20733033
Am J Respir Crit Care Med. 2014 Apr 1;189(7):799-811
pubmed: 24555512
J Immunol. 2013 Feb 1;190(3):1217-26
pubmed: 23267025
Nature. 2011 Sep 14;477(7366):596-600
pubmed: 21918512
J Exp Med. 2016 May 2;213(5):647-56
pubmed: 27114610
Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7326-7337
pubmed: 32170015
J Immunol. 2018 Mar 1;200(5):1817-1828
pubmed: 29374076
Proc Natl Acad Sci U S A. 2021 Jul 13;118(28):
pubmed: 34260403
Eur J Immunol. 2007 Nov;37(11):3030-9
pubmed: 17935074
Infect Immun. 2014 Oct;82(10):4204-11
pubmed: 25069982
Nature. 2006 Mar 9;440(7081):237-41
pubmed: 16407889
Blood Adv. 2022 Apr 12;6(7):2001-2013
pubmed: 34991159
Sci Immunol. 2018 Aug 24;3(26):
pubmed: 30143555
Sci Adv. 2020 Nov 18;6(47):
pubmed: 33208362
Nat Commun. 2020 May 5;11(1):2212
pubmed: 32371889
Cell Rep. 2014 Jul 24;8(2):570-82
pubmed: 25043180
Elife. 2017 Jun 02;6:
pubmed: 28574339
Nat Rev Microbiol. 2009 Sep;7(9):654-65
pubmed: 19680249
Proc Natl Acad Sci U S A. 2016 Dec 13;113(50):14384-14389
pubmed: 27911804
J Cell Biol. 2010 Nov 1;191(3):677-91
pubmed: 20974816
Blood. 2015 Mar 19;125(12):1948-56
pubmed: 25624317
Eur J Immunol. 2018 Apr;48(4):584-592
pubmed: 29274245
Front Immunol. 2019 Jul 18;10:1670
pubmed: 31379861
Sci Rep. 2018 Sep 19;8(1):14047
pubmed: 30232373
J Clin Invest. 2013 Apr;123(4):1630-7
pubmed: 23478406
Cell Rep. 2014 Aug 7;8(3):883-96
pubmed: 25066128
Immunity. 2017 Mar 21;46(3):421-432
pubmed: 28314592
Cell. 2021 Aug 19;184(17):4480-4494.e15
pubmed: 34320407
Proc Natl Acad Sci U S A. 2014 May 20;111(20):7403-8
pubmed: 24803432
Cell Host Microbe. 2019 Apr 10;25(4):526-536.e4
pubmed: 30930127
Nature. 2021 May;593(7860):607-611
pubmed: 33883744
Cell Death Dis. 2019 Apr 11;10(4):326
pubmed: 30975978
Cell Rep. 2021 Mar 23;34(12):108887
pubmed: 33761363
Nature. 2016 Mar 24;531(7595):513-7
pubmed: 26982733
Sci Signal. 2021 Mar 09;14(673):
pubmed: 33688080
J Exp Med. 2016 Sep 19;213(10):2113-28
pubmed: 27573815
J Exp Med. 2007 Dec 24;204(13):3235-45
pubmed: 18070936
Immunity. 2018 Jan 16;48(1):35-44.e6
pubmed: 29195811
Front Immunol. 2021 May 28;12:683803
pubmed: 34122445
Science. 2004 Mar 5;303(5663):1532-5
pubmed: 15001782
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2562-7
pubmed: 18256184
Cell. 1998 Feb 20;92(4):501-9
pubmed: 9491891
Nat Methods. 2011 Jan;8(1):91-6
pubmed: 21151136
Sci Signal. 2018 Sep 04;11(546):
pubmed: 30181240
J Exp Med. 2016 May 2;213(5):657-65
pubmed: 27045008
Cell Host Microbe. 2017 May 10;21(5):611-618.e5
pubmed: 28494242
Infect Immun. 2013 Jun;81(6):2043-52
pubmed: 23529619
Cell. 1995 Feb 10;80(3):401-11
pubmed: 7859282
J Cell Biol. 2009 Jan 26;184(2):205-13
pubmed: 19153223
Nature. 2002 May 2;417(6884):91-4
pubmed: 12018205
JCI Insight. 2018 Feb 8;3(3):
pubmed: 29415887
Proc Natl Acad Sci U S A. 2005 May 31;102(22):8006-11
pubmed: 15911752
Mucosal Immunol. 2017 May;10(3):757-774
pubmed: 27554297
Nature. 2021 Mar;591(7848):131-136
pubmed: 33472215
PLoS One. 2013;8(1):e54205
pubmed: 23342104
Toxins (Basel). 2021 Dec 09;13(12):
pubmed: 34941717
Cell Death Discov. 2018 Jun 27;4:3
pubmed: 30062052
Annu Rev Cell Dev Biol. 2020 Oct 6;36:191-218
pubmed: 32663035
Sci Immunol. 2019 Oct 18;4(40):
pubmed: 31628160
Nat Struct Mol Biol. 2004 Aug;11(8):777-83
pubmed: 15247907
Nat Commun. 2018 Sep 14;9(1):3767
pubmed: 30218080
J Innate Immun. 2017;9(3):250-261
pubmed: 28222444
Cell Death Differ. 2018 Mar;25(3):486-541
pubmed: 29362479
J Immunol. 2021 Apr 15;206(8):1913-1922
pubmed: 33712519
J Cell Mol Med. 2008 Sep-Oct;12(5A):1767-76
pubmed: 18081695
Sci Immunol. 2018 Aug 24;3(26):
pubmed: 30143554