Structural basis for the activation and ligand recognition of the human oxytocin receptor.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
18 07 2022
Historique:
received: 25 02 2022
accepted: 10 06 2022
entrez: 19 7 2022
pubmed: 20 7 2022
medline: 22 7 2022
Statut: epublish

Résumé

The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction. Here we report the single-particle cryo-electron microscopy structure of the active oxytocin receptor (OTR) in complex with its cognate ligand oxytocin. Our structure provides high-resolution insights into the OT binding mode, the OTR activation mechanism as well as the subtype specificity within the oxytocin/vasopressin receptor family.

Identifiants

pubmed: 35851571
doi: 10.1038/s41467-022-31325-0
pii: 10.1038/s41467-022-31325-0
pmc: PMC9293896
doi:

Substances chimiques

Ligands 0
Receptors, Oxytocin 0
Receptors, Vasopressin 0
Oxytocin 50-56-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4153

Informations de copyright

© 2022. The Author(s).

Références

J Endocrinol. 1998 Feb;156(2):223-9
pubmed: 9518866
Nat Struct Mol Biol. 2022 Mar;29(3):274-281
pubmed: 35241813
Sci Adv. 2021 May 21;7(21):
pubmed: 34020960
J Chem Theory Comput. 2014 Nov 11;10(11):5047-5056
pubmed: 25400521
Science. 2008 Nov 7;322(5903):900-4
pubmed: 18988842
Clin Obstet Gynecol. 1995 Jun;38(2):232-45
pubmed: 7554591
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
pubmed: 22505256
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Sci Adv. 2020 Jul 15;6(29):eabb5419
pubmed: 32832646
Methods Mol Biol. 2019;2001:235-271
pubmed: 31134574
PLoS Biol. 2021 Jun 4;19(6):e3001295
pubmed: 34086670
Cell Res. 2021 Aug;31(8):932-934
pubmed: 33664408
Protein Sci. 2018 Jan;27(1):293-315
pubmed: 29067766
Biol Psychiatry. 2016 Feb 1;79(3):213-21
pubmed: 26208744
Science. 2019 May 10;364(6440):552-557
pubmed: 31073061
J Biol Chem. 2012 Feb 3;287(6):3617-29
pubmed: 22069312
J Med Chem. 1999 Apr 8;42(7):1312-9
pubmed: 10197974
Nat Protoc. 2022 Mar;17(3):698-726
pubmed: 35140409
Sci Rep. 2021 Apr 21;11(1):8630
pubmed: 33883583
Biol Psychiatry. 2016 Feb 1;79(3):234-42
pubmed: 26257243
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
PLoS One. 2017 Apr 20;12(4):e0175642
pubmed: 28426733
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
Nat Struct Mol Biol. 2018 Dec;25(12):1086-1092
pubmed: 30455434
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Nat Chem Biol. 2020 May;16(5):507-512
pubmed: 32152538
Schizophr Res. 2011 Oct;132(1):50-3
pubmed: 21840177
Nat Commun. 2020 Jun 15;11(1):3031
pubmed: 32541785
J Med Chem. 1966 Jan;9(1):55-7
pubmed: 6006226
J Biol Chem. 1995 Oct 27;270(43):25771-7
pubmed: 7592759
J Med Chem. 2018 Oct 11;61(19):8670-8692
pubmed: 30199637
EMBO J. 1995 May 15;14(10):2176-82
pubmed: 7774575
Cell Res. 2021 Aug;31(8):929-931
pubmed: 33742150
Nat Commun. 2021 Feb 5;12(1):815
pubmed: 33547286
Nat Commun. 2018 Sep 13;9(1):3712
pubmed: 30213947
Nature. 2015 Aug 13;524(7564):173-179
pubmed: 26147082
Biochemistry. 2002 Apr 23;41(16):5086-92
pubmed: 11955056
Sci Adv. 2021 Dec 10;7(50):eabk2872
pubmed: 34878828
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Methods Mol Biol. 2022;2384:153-174
pubmed: 34550574

Auteurs

Yann Waltenspühl (Y)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
Novo Nordisk A/S, Novo Nordisk Park 1, DK-2760, Måløv, Denmark.

Janosch Ehrenmann (J)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
leadXpro AG, PARK innovAARE, CH-5234, Villigen, Switzerland.

Santiago Vacca (S)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.

Cristian Thom (C)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.

Ohad Medalia (O)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.

Andreas Plückthun (A)

Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland. plueckthun@bioc.uzh.ch.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH