Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.
cell biology
clathrin-coated vesicle
endocytosis
human
intracellular trafficking
pinchase
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 07 2022
19 07 2022
Historique:
received:
24
03
2022
accepted:
18
07
2022
pubmed:
20
7
2022
medline:
2
8
2022
entrez:
19
7
2022
Statut:
epublish
Résumé
The formation of a clathrin-coated vesicle (CCV) is a major membrane remodeling process that is crucial for membrane traffic in cells. Besides clathrin, these vesicles contain at least 100 different proteins although it is unclear how many are essential for the formation of the vesicle. Here, we show that intracellular clathrin-coated formation can be induced in living cells using minimal machinery and that it can be achieved on various membranes, including the mitochondrial outer membrane. Chemical heterodimerization was used to inducibly attach a clathrin-binding fragment 'hook' to an 'anchor' protein targeted to a specific membrane. Endogenous clathrin assembled to form coated pits on the mitochondria, termed MitoPits, within seconds of induction. MitoPits are double-membraned invaginations that form preferentially on high curvature regions of the mitochondrion. Upon induction, all stages of CCV formation - initiation, invagination, and even fission - were faithfully reconstituted. We found no evidence for the functional involvement of accessory proteins in this process. In addition, fission of MitoPit-derived vesicles was independent of known scission factors including dynamins and dynamin-related protein 1 (Drp1), suggesting that the clathrin cage generates sufficient force to bud intracellular vesicles. Our results suggest that, following its recruitment, clathrin is sufficient for intracellular CCV formation.
Identifiants
pubmed: 35852853
doi: 10.7554/eLife.78929
pii: 78929
pmc: PMC9337851
doi:
pii:
Substances chimiques
Clathrin
0
Dynamins
EC 3.6.5.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, Küey et al.
Déclaration de conflit d'intérêts
CK, MS, GL, MH, SR No competing interests declared
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