Real-Time Polymerase Chain Reaction for the Detection of
Humans
Clarithromycin
/ pharmacology
Helicobacter pylori
/ genetics
Anti-Bacterial Agents
/ pharmacology
Real-Time Polymerase Chain Reaction
/ methods
RNA, Ribosomal, 23S
/ genetics
Prospective Studies
Drug Resistance, Bacterial
/ genetics
Helicobacter Infections
/ diagnosis
Microbial Sensitivity Tests
Clarithromycin resistance
Culture
Rapid urease test
Real-time polymerase chain reaction
Journal
Gut and liver
ISSN: 2005-1212
Titre abrégé: Gut Liver
Pays: Korea (South)
ID NLM: 101316452
Informations de publication
Date de publication:
15 05 2023
15 05 2023
Historique:
received:
24
02
2022
revised:
30
03
2022
accepted:
14
04
2022
medline:
16
5
2023
pubmed:
21
7
2022
entrez:
20
7
2022
Statut:
ppublish
Résumé
Real-time polymerase chain reaction (RT-PCR) is a fast and simple method for the simultaneous detection of clarithromycin (CLR) resistance and A total of 70 specimens with confirmed Among the 70 specimens analyzed, the positivity rate was 97.1% (68/70) with RT-PCR and 82.9% (58/70) with RUT. CLR resistance (minimum inhibitory concentration >1.0 μg/mL) was confirmed in 18.6% (13/70), and fluorescence melting curve analysis showed that 84.6% (11/13) had point mutations in 23S rRNA. Ten specimens had only A2143G mutation, and one specimen contained both A2142G and A2143G mutations. RT-PCR assay was found to be more efficient than RUT in detecting
Sections du résumé
Background/Aims
Real-time polymerase chain reaction (RT-PCR) is a fast and simple method for the simultaneous detection of clarithromycin (CLR) resistance and
Methods
A total of 70 specimens with confirmed
Results
Among the 70 specimens analyzed, the positivity rate was 97.1% (68/70) with RT-PCR and 82.9% (58/70) with RUT. CLR resistance (minimum inhibitory concentration >1.0 μg/mL) was confirmed in 18.6% (13/70), and fluorescence melting curve analysis showed that 84.6% (11/13) had point mutations in 23S rRNA. Ten specimens had only A2143G mutation, and one specimen contained both A2142G and A2143G mutations.
Conclusions
RT-PCR assay was found to be more efficient than RUT in detecting
Identifiants
pubmed: 35854654
pii: gnl220076
doi: 10.5009/gnl220076
pmc: PMC10191795
doi:
Substances chimiques
Clarithromycin
H1250JIK0A
Anti-Bacterial Agents
0
RNA, Ribosomal, 23S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-381Commentaires et corrections
Type : CommentIn
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