A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy.
Journal
Nature chemistry
ISSN: 1755-4349
Titre abrégé: Nat Chem
Pays: England
ID NLM: 101499734
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
01
10
2021
accepted:
09
06
2022
pubmed:
22
7
2022
medline:
31
8
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
The controlled switching of fluorophores between non-fluorescent and fluorescent states is central to every super-resolution fluorescence microscopy (nanoscopy) technique, and the exploration of radically new switching mechanisms remains critical to boosting the performance of established, as well as emerging super-resolution methods. Photoactivatable dyes offer substantial improvements to many of these techniques, but often rely on photolabile protecting groups that limit their applications. Here we describe a general method to transform 3,6-diaminoxanthones into caging-group-free photoactivatable fluorophores. These photoactivatable xanthones (PaX) assemble rapidly and cleanly into highly fluorescent, photo- and chemically stable pyronine dyes upon irradiation with light. The strategy is extendable to carbon- and silicon-bridged xanthone analogues, yielding a family of photoactivatable labels spanning much of the visible spectrum. Our results demonstrate the versatility and utility of PaX dyes in fixed and live-cell labelling for conventional microscopy, as well as the coordinate-stochastic and deterministic nanoscopies STED, PALM and MINFLUX.
Identifiants
pubmed: 35864152
doi: 10.1038/s41557-022-00995-0
pii: 10.1038/s41557-022-00995-0
pmc: PMC9417988
doi:
Substances chimiques
Fluorescent Dyes
0
Ionophores
0
Silicon
Z4152N8IUI
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1013-1020Informations de copyright
© 2022. The Author(s).
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