14-3-3-zeta mediates GLP-1 receptor agonist action to alter α cell proglucagon processing.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
22 Jul 2022
Historique:
entrez: 22 7 2022
pubmed: 23 7 2022
medline: 23 7 2022
Statut: ppublish

Résumé

Recent studies demonstrate that α cells contribute to glucose-stimulated insulin secretion (GSIS). Glucagon-like peptide-1 receptor (GLP-1R) agonists potently potentiate GSIS, making these drugs useful for diabetes treatment. However, the role of α and β cell paracrine interactions in the effects of GLP-1R agonists is undefined. We previously found that increased β cell GLP-1R signaling activates α cell GLP-1 expression. Here, we characterized the bidirectional paracrine cross-talk by which α and β cells communicate to mediate the effects of the GLP-1R agonist, liraglutide. We find that the effect of liraglutide to enhance GSIS is blunted by α cell ablation in male mice. Furthermore, the effect of β cell GLP-1R signaling to activate α cell GLP-1 is mediated by a secreted protein factor that is regulated by the signaling protein, 14-3-3-zeta, in mouse and human islets. These data refine our understanding of GLP-1 pharmacology and identify 14-3-3-zeta as a potential target to enhance α cell GLP-1 production.

Identifiants

pubmed: 35867787
doi: 10.1126/sciadv.abn3773
pmc: PMC9307243
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabn3773

Subventions

Organisme : NIDDK NIH HHS
ID : F30 DK126538
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK020541
Pays : United States
Organisme : NIH HHS
ID : T35 OD010941
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK098085
Pays : United States

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Auteurs

Marlena M Holter (MM)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.

Daryl J Phuong (DJ)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.

Isaac Lee (I)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.

Mridusmita Saikia (M)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.
Nancy E. and Peter C. Meinig School of Biomedical Engineering, Ithaca, NY, USA.

Lisa Weikert (L)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.

Samantha Fountain (S)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.

Elizabeth T Anderson (ET)

Proteomics and Metabolomics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY, USA.

Qin Fu (Q)

Proteomics and Metabolomics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY, USA.

Sheng Zhang (S)

Proteomics and Metabolomics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY, USA.

Kyle W Sloop (KW)

Diabetes and Complications, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN, USA.

Bethany P Cummings (BP)

Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY, USA.
Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California, Davis, Sacramento, CA, USA.

Classifications MeSH