3D bioprinting of modified mannan bioink for tissue engineering.
Biotechnology and bioengineering
Material sciences
Tissue Engineering
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
16 09 2022
16 09 2022
Historique:
entrez:
26
7
2022
pubmed:
27
7
2022
medline:
28
7
2022
Statut:
epublish
Résumé
This protocol details the steps for preparation of a recently developed bioink, named YM-MA, which is based on methacrylate anhydride-modified yeast mannan. A light-assisted 3D bioprinting is performed to analyze the printability of YM-MA bioink. We describe how cell experiments, animal models of subcutaneous implantation in a Sprague Dawley rat model, and nude mice are used to evaluate the cytocompatibility, histocompatibility, and chondrogenesis of YM-MA bioink. This protocol provides a versatile strategy to develop bioinks of polysaccharides with chemical modification sites such as hydroxyl group. For complete details on the use and execution of this protocol, please refer to Huang et al. (2021).
Identifiants
pubmed: 35880129
doi: 10.1016/j.xpro.2022.101585
pii: S2666-1667(22)00465-8
pmc: PMC9307568
pii:
doi:
Substances chimiques
Mannans
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101585Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
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pubmed: 32314911
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pubmed: 28928062