Melatonin Reverses the Warburg-Type Metabolism and Reduces Mitochondrial Membrane Potential of Ovarian Cancer Cells Independent of MT1 Receptor Activation.
SKOV-3 cells
Warburg effect
glucose
melatonin
mitochondrial metabolism
ovarian cancer
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
07 Jul 2022
07 Jul 2022
Historique:
received:
29
05
2022
revised:
29
06
2022
accepted:
01
07
2022
entrez:
27
7
2022
pubmed:
28
7
2022
medline:
29
7
2022
Statut:
epublish
Résumé
Ovarian cancer (OC) is the most lethal gynecologic malignancy, and melatonin has shown various antitumor properties. Herein, we investigated the influence of melatonin therapy on energy metabolism and mitochondrial integrity in SKOV-3 cells and tested whether its effects depended on MT1 receptor activation. SKOV-3 cells were exposed to different melatonin concentrations, and experimental groups were divided as to the presence of MT1 receptors (melatonin groups) or receptor absence by RNAi silencing (siRNA MT1+melatonin). Intracellular melatonin levels increased after treatment with melatonin independent of the MT1. The mitochondrial membrane potential of SKOV-3 cells decreased in the group treated with the highest melatonin concentration. Melatonin reduced cellular glucose consumption, while MT1 knockdown increased its consumption. Interconversion of lactate to pyruvate increased after treatment with melatonin and was remarkable in siRNA MT1 groups. Moreover, lactate dehydrogenase activity decreased with melatonin and increased after MT1 silencing at all concentrations. The UCSC XenaBrowser tool showed a positive correlation between the human
Identifiants
pubmed: 35889222
pii: molecules27144350
doi: 10.3390/molecules27144350
pmc: PMC9321770
pii:
doi:
Substances chimiques
MTNR1A protein, human
0
Pyruvates
0
RNA, Small Interfering
0
Receptor, Melatonin, MT1
0
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : São Paulo Research Foundation
ID : 2019/00906-6
Organisme : São Paulo Research Foundation
ID : 2018/15797-5
Organisme : National Council for Scientific and Technological Development
ID : 304108/2020-0
Organisme : Coordenação de Aperfeicoamento de Pessoal de Nível Superior
ID : 001
Références
Cancer Lett. 2020 Apr 28;476:34-47
pubmed: 32061949
Molecules. 2018 Feb 26;23(3):
pubmed: 29495398
CA Cancer J Clin. 2022 Jan;72(1):7-33
pubmed: 35020204
Int J Mol Sci. 2017 Jul 26;18(8):
pubmed: 28933733
Int J Mol Sci. 2021 Nov 19;22(22):
pubmed: 34830375
J Ovarian Res. 2019 Mar 26;12(1):26
pubmed: 30914056
J Pineal Res. 2015 Mar;58(2):234-50
pubmed: 25612238
Molecules. 2021 Dec 02;26(23):
pubmed: 34885890
Front Oncol. 2021 Jan 13;10:600113
pubmed: 33520711
Lasers Med Sci. 2016 Nov;31(8):1565-1572
pubmed: 27365110
J Proteome Res. 2016 Oct 7;15(10):3872-3882
pubmed: 27604906
Future Med Chem. 2021 Feb;13(4):335-339
pubmed: 33399498
Med Pharm Rep. 2019 Jul;92(3):226-234
pubmed: 31460502
PLoS One. 2015 Aug 07;10(8):e0135420
pubmed: 26252771
Pharmacol Res. 2012 Apr;65(4):437-44
pubmed: 22311380
Int J Mol Sci. 2016 Dec 16;17(12):
pubmed: 27999288
Nucleic Acids Res. 2017 Jul 3;45(W1):W98-W102
pubmed: 28407145
Antioxidants (Basel). 2021 Feb 01;10(2):
pubmed: 33535472
J Diabetes Res. 2015;2015:538529
pubmed: 26295055
Int J Mol Sci. 2018 Aug 18;19(8):
pubmed: 30126181
Cancers (Basel). 2019 May 29;11(6):
pubmed: 31146503
J Biotechnol. 2011 Apr 20;153(1-2):27-34
pubmed: 21392546
Int J Cancer. 2021 Jun 1;148(11):2848-2856
pubmed: 33586202
Lancet. 2019 Mar 23;393(10177):1240-1253
pubmed: 30910306
Fertil Steril. 2009 Jul;92(1):328-43
pubmed: 18804205
Front Endocrinol (Lausanne). 2019 Apr 17;10:249
pubmed: 31057485
Biochim Biophys Acta. 2002 Feb 15;1553(3):188-211
pubmed: 11997128
Front Physiol. 2017 Feb 21;8:97
pubmed: 28270771
Nat Biotechnol. 2020 Jun;38(6):675-678
pubmed: 32444850
Cell Mol Life Sci. 2014 Aug;71(16):2997-3025
pubmed: 24554058
Physiology (Bethesda). 2020 Mar 1;35(2):86-95
pubmed: 32024428
Mol Oncol. 2019 Feb;13(2):358-375
pubmed: 30443978
J Res Med Sci. 2017 Jan 27;22:2
pubmed: 28400824
J Cell Physiol. 2021 Jan;236(1):27-40
pubmed: 32725819
In Vivo. 2017 Sep-Oct;31(5):801-809
pubmed: 28882945
Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E7997-E8006
pubmed: 28874589
Int J Mol Sci. 2013 Mar 25;14(4):6597-613
pubmed: 23528889
Clin Exp Optom. 2019 Mar;102(2):99-108
pubmed: 30074278
Life Sci. 2022 Apr 1;294:120352
pubmed: 35074409
Cytotechnology. 2010 Dec;62(6):585-94
pubmed: 21069459
J Cancer Res Clin Oncol. 2020 Aug;146(8):1893-1922
pubmed: 32583237
Steroids. 2017 Feb;118:93-108
pubmed: 28041951
J Pineal Res. 2017 May;62(4):
pubmed: 28099762
Cell Mol Life Sci. 2019 Mar;76(5):837-863
pubmed: 30430198
PLoS One. 2017 Jul 21;12(7):e0180953
pubmed: 28732061
Cell Mol Life Sci. 2020 Jul;77(13):2527-2542
pubmed: 31970423
Endocr Relat Cancer. 2015 Jun;22(3):R183-204
pubmed: 25876649