Actin nano-architecture of phagocytic podosomes.

Actins / chemistry Microscopy Myosin Type II Podosomes Talin / chemistry

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
27 07 2022
Historique:
received: 10 01 2022
accepted: 13 07 2022
entrez: 27 7 2022
pubmed: 28 7 2022
medline: 30 7 2022
Statut: epublish

Résumé

Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterize the structure and organization of phagocytic podosomes using interferometric photoactivated localization microscopy, a super-resolution microscopy technique capable of 15-20 nm resolution, together with structured illumination microscopy and localization-based super-resolution microscopy. Phagocytic podosomes are observed during frustrated phagocytosis, a model in which cells attempt to engulf micropatterned IgG antibodies. For circular patterns, this results in regular arrays of podosomes with well-defined geometry. Using persistent homology, we develop a pipeline for semi-automatic identification and measurement of podosome features. These studies reveal an hourglass shape of the podosome actin core, a protruding knob at the bottom of the core, and two actin networks extending from the core. Additionally, the distributions of paxillin, talin, myosin II, α-actinin, cortactin, and microtubules relative to actin are characterized.

Identifiants

DOI: 10.1038/s41467-022-32038-0 PMID: 35896550 PMC: PMC9329332
pubmed: 35896550
doi: 10.1038/s41467-022-32038-0
pii: 10.1038/s41467-022-32038-0
pmc: PMC9329332
doi:

Substances chimiques

Actins 0
Talin 0
Myosin Type II EC 3.6.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

4363

Subventions

Organisme : NIGMS NIH HHS
ID : T32 GM008570
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127145
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122596
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM067553
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB018816
Pays : United States
Organisme : NINDS NIH HHS
ID : P30 NS045892
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD079124
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

J Cody Herron (JC)

Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Computational Medicine Program, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
ORCID: 0000-0001-6240-9950

Shiqiong Hu (S)

Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Takashi Watanabe (T)

Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Division of Gene Regulation, Cancer Center, Fujita Health University, Toyoake, Aichi, Japan.

Ana T Nogueira (AT)

Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Bei Liu (B)

Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
ORCID: 0000-0001-5979-3082

Megan E Kern (ME)

Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Jesse Aaron (J)

Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA, USA.
ORCID: 0000-0002-7543-9590

Aaron Taylor (A)

Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA, USA.

Michael Pablo (M)

Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Program in Molecular and Cellular Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
ORCID: 0000-0001-9738-8109

Teng-Leong Chew (TL)

Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA, USA.
ORCID: 0000-0002-3139-7560

Timothy C Elston (TC)

Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. telston@med.unc.edu.
Computational Medicine Program, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. telston@med.unc.edu.
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. telston@med.unc.edu.
ORCID: 0000-0002-4601-2117

Klaus M Hahn (KM)

Computational Medicine Program, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. khahn@med.unc.edu.
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. khahn@med.unc.edu.
ORCID: 0000-0002-1970-7562

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Actines › Actin nano-architecture of phagocytic podosomes.
questionsmedicales.fr › Disciplines des sciences naturelles › Physique › Microscopie › Actin nano-architecture of phagocytic podosomes.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Myosines › Myosine de type II › Actin nano-architecture of phagocytic podosomes.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Podosomes › Actin nano-architecture of phagocytic podosomes.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Taline › Actin nano-architecture of phagocytic podosomes.