Environmental DNA methylation of Lymnaea stagnalis varies with age and is hypermethylated compared to tissue DNA.
age assessment
aquatic biomonitoring
eDNA methylation
eDNA release mechanism
environmental DNA
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
revised:
12
07
2022
received:
21
01
2022
accepted:
25
07
2022
pubmed:
29
7
2022
medline:
7
12
2022
entrez:
28
7
2022
Statut:
ppublish
Résumé
Environmental DNA (eDNA) approaches contributing to species identifications are quickly becoming the new norm in biomonitoring and ecosystem assessments. Yet, information such as age and health state of the population, which is vital to species biomonitoring, has not been accessible from eDNA. DNA methylation has the potential to provide such information on the state of a population. Here, we measured the methylation of eDNA along with tissue DNA (tDNA) of Lymnaea stagnalis at four life stages. We demonstrate that eDNA methylation varies with age and allows distinguishing among age classes. Moreover, eDNA was globally hypermethylated in comparison to tDNA. This difference was age-specific and connected to a limited number of eDNA sites. This differential methylation pattern suggests that eDNA release with age is partially regulated through DNA methylation. Our findings help to understand mechanisms involved in eDNA release and shows the potential of eDNA methylation analysis to assess age classes. Such age class assessments will encourage future eDNA studies to assess fundamental processes of population dynamics and functioning in ecology, biodiversity conservation and impact assessments.
Identifiants
pubmed: 35899418
doi: 10.1111/1755-0998.13691
pmc: PMC10087510
doi:
Substances chimiques
DNA, Environmental
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-91Informations de copyright
© 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
Références
Mol Ecol Resour. 2019 Nov;19(6):1486-1496
pubmed: 31349392
Genome Res. 2013 Sep;23(9):1522-40
pubmed: 23804400
Environ Sci Technol. 2014;48(3):1819-27
pubmed: 24422450
PLoS One. 2014 Dec 05;9(12):e114639
pubmed: 25479160
Environ Sci Technol. 2019 Oct 15;53(20):11849-11859
pubmed: 31545591
Lancet. 2018 Sep 1;392(10149):777-786
pubmed: 30100054
Sci Total Environ. 2021 Nov 25;797:149175
pubmed: 34303977
BMC Bioinformatics. 2011 Jan 26;12:35
pubmed: 21269502
PLoS One. 2014 Jan 14;9(1):e86232
pubmed: 24454962
Mutat Res Genet Toxicol Environ Mutagen. 2016 Jan 15;796:46-53
pubmed: 26778509
Science. 1980 Nov 7;210(4470):604-10
pubmed: 6254144
Exp Mol Med. 2017 Apr 28;49(4):e322
pubmed: 28450738
Commun Biol. 2019 Dec 10;2:461
pubmed: 31840106
Nat Commun. 2021 Mar 12;12(1):1615
pubmed: 33712580
Aging (Albany NY). 2020 Dec 3;12(23):24057-24080
pubmed: 33276343
Appl Microbiol Biotechnol. 2018 Jun;102(12):5045-5063
pubmed: 29713790
Clin Exp Med. 2018 Feb;18(1):1-14
pubmed: 28752221
Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):11724-11729
pubmed: 30373831
Mol Ecol Resour. 2014 Sep;14(5):976-87
pubmed: 24606053
J Genet Eng Biotechnol. 2019 Oct 14;17(1):6
pubmed: 31659568
Brief Bioinform. 2019 Mar 25;20(2):585-597
pubmed: 29672679
Trends Ecol Evol. 2014 Jun;29(6):358-67
pubmed: 24821515
Biodivers Data J. 2020 Jan 14;8:e39679
pubmed: 31992945
Aging (Albany NY). 2017 Mar 28;9(3):1055-1068
pubmed: 28373601
Clin Epigenetics. 2020 Nov 4;12(1):167
pubmed: 33148325
Ecol Appl. 2022 Jul;32(5):e2614
pubmed: 35365955
Environ Health Perspect. 2020 Jun;128(6):67003
pubmed: 32484729
Nat Aging. 2023 Sep;3(9):1144-1166
pubmed: 37563227
Genome Biol. 2017 Apr 11;18(1):68
pubmed: 28399939
PLoS One. 2017 Nov 2;12(11):e0187636
pubmed: 29095959
PLoS One. 2017 May 17;12(5):e0176541
pubmed: 28520733
Nature. 2000 Nov 30;408(6812):538-40
pubmed: 11117732
Evol Appl. 2021 Sep 23;14(9):2305-2318
pubmed: 34603500
Endocr Relat Cancer. 2019 Jul;26(7):R415-R439
pubmed: 31035251
Sci Total Environ. 2020 Feb 20;704:135314
pubmed: 31780169
Brief Bioinform. 2018 Nov 27;19(6):1415-1429
pubmed: 28481971
Integr Comp Biol. 2014 Jul;54(1):77-86
pubmed: 24785828
Mol Ecol Resour. 2018 Nov;18(6):1374-1380
pubmed: 29981199
Genome Biol. 2012 Oct 03;13(10):R87
pubmed: 23034086
Bioinformatics. 2011 Jan 15;27(2):225-31
pubmed: 21098430
Environ Sci Technol. 2016 Oct 4;50(19):10456-10464
pubmed: 27580258
Evodevo. 2020 Dec 4;11(1):24
pubmed: 33292457
Prog Mol Biol Transl Sci. 2018;157:175-232
pubmed: 29933950
Epigenetics. 2019 Jan;14(1):1-15
pubmed: 30574831
Epigenomics. 2013 Jun;5(3):341-8
pubmed: 23750648
Nucleic Acids Res. 2005 Oct 13;33(18):5868-77
pubmed: 16224102
Biomed Res Int. 2021 Mar 20;2021:8827516
pubmed: 33824878
PLoS One. 2019 Apr 24;14(4):e0215586
pubmed: 31017960
PLoS One. 2019 Aug 27;14(8):e0221641
pubmed: 31454376
Epigenetics. 2021 Jan;16(1):45-53
pubmed: 32614650
Elife. 2020 Jun 16;9:
pubmed: 32539932
Mol Ecol. 2017 Nov;26(21):5872-5895
pubmed: 28921802
Nat Rev Clin Oncol. 2018 Jul;15(7):459-466
pubmed: 29666440
Genomics. 2020 Nov;112(6):4567-4576
pubmed: 32712292
Pediatr Res. 2016 Jan;79(1-2):212-9
pubmed: 26466079
Genome Res. 2007 May;17(5):625-31
pubmed: 17420183
PLoS One. 2011 Jan 21;6(1):e16229
pubmed: 21283691
Essays Biochem. 2019 Dec 20;63(6):717-726
pubmed: 31782496
Nat Commun. 2018 Nov 29;9(1):5068
pubmed: 30498206
Nat Rev Genet. 2013 Mar;14(3):204-20
pubmed: 23400093
Genome Biol. 2013;14(10):R115
pubmed: 24138928
Curr Opin Biotechnol. 2016 Apr;38:143-9
pubmed: 26901403
Commun Biol. 2021 May 31;4(1):642
pubmed: 34059764
Mol Ecol. 2021 Jul;30(13):3057-3067
pubmed: 32608023
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5237-42
pubmed: 10805783
Mol Ecol Resour. 2023 Jan;23(1):81-91
pubmed: 35899418