Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore.
Bacillus anthracis
exosporium
interspace
polysaccharide
spore
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
19
07
2022
received:
09
02
2022
accepted:
22
07
2022
pubmed:
29
7
2022
medline:
17
9
2022
entrez:
28
7
2022
Statut:
ppublish
Résumé
A fundamental question in cell biology is how cells assemble their outer layers. The bacterial endospore is a well-established model for cell layer assembly. However, the assembly of the exosporium, a complex protein shell comprising the outermost layer in the pathogen Bacillus anthracis, remains poorly understood. Exosporium assembly begins with the deposition of proteins at one side of the spore surface, followed by the progressive encirclement of the spore. We seek to resolve a major open question: the mechanism directing exosporium assembly to the spore, and then into a closed shell. We hypothesized that material directly underneath the exosporium (the interspace) directs exosporium assembly to the spore and drives encirclement. In support of this, we show that the interspace possesses at least two distinct layers of polysaccharide. Secondly, we show that putative polysaccharide biosynthetic genes are required for exosporium encirclement, suggesting a direct role for the interspace. These results not only significantly clarify the mechanism of assembly of the exosporium, an especially widespread bacterial outer layer, but also suggest a novel mechanism in which polysaccharide layers drive the assembly of a protein shell.
Identifiants
pubmed: 35900297
doi: 10.1111/mmi.14966
pmc: PMC9549345
mid: NIHMS1827214
doi:
Substances chimiques
Bacterial Proteins
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
258-277Subventions
Organisme : NIAID NIH HHS
ID : R01 AI093493
Pays : United States
Informations de copyright
© 2022 John Wiley & Sons Ltd.
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