An atypical MAPK regulates translocation of a GATA transcription factor in response to chemoattractant stimulation.
Dictyostelium
Chemotaxis
Folate
GATA transcription factor
Kinase translocation reporter
MAPK
Nuclear-to-cytoplasmic translocation
Transcription factor
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
15 08 2022
15 08 2022
Historique:
received:
20
04
2022
accepted:
25
07
2022
pubmed:
3
8
2022
medline:
23
8
2022
entrez:
2
8
2022
Statut:
ppublish
Résumé
The Dictyostelium atypical mitogen-activated protein kinase (MAPK) Erk2 is required for chemotactic responses to cAMP as amoeba undergo multicellular development. In this study, Erk2 was found to be essential for the cAMP-stimulated translocation of the GATA transcription factor GtaC as indicated by the distribution of a GFP-GtaC reporter. Erk2 was also found to be essential for the translocation of GtaC in response to external folate, a foraging signal that directs the chemotaxis of amoeba to bacteria. Erk1, the only other Dictyostelium MAPK, was not required for the GtaC translocation to either chemoattractant, indicating that GFP-GtaC is a kinase translocation reporter specific for atypical MAPKs. The translocation of GFP-GtaC in response to folate was absent in mutants lacking the folate receptor Far1 or the coupled G-protein subunit Gα4. Loss of GtaC function resulted in enhanced chemotactic movement to folate, suggesting that GtaC suppresses responses to folate. The alteration of four Erk2-preferred phosphorylation sites in GtaC impacted the translocation of GFP-GtaC in response to folate and the GFP-GtaC-mediated rescue of aggregation and development of gtaC- cells. The ability of different chemoattractants to stimulate Erk2-regulated GtaC translocation suggests that atypical MAPK-mediated regulation of transcription factors can contribute to different cell fates.
Identifiants
pubmed: 35916164
pii: 276183
doi: 10.1242/jcs.260148
pmc: PMC9481928
pii:
doi:
Substances chimiques
Chemotactic Factors
0
GATA Transcription Factors
0
Folic Acid
935E97BOY8
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM131269
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM140953
Pays : United States
Organisme : National Natural Science Foundation of China
ID : 32170701
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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