Identification of Intestinal NaCl Absorptive-Anion Secretory Cells: Potential Functional Significance.
CFTR
DRA
NHE3
Na absorption
anion secretion
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2022
2022
Historique:
received:
08
03
2022
accepted:
18
05
2022
entrez:
5
8
2022
pubmed:
6
8
2022
medline:
6
8
2022
Statut:
epublish
Résumé
Use of human enteroids studied in the undifferentiated and differentiated state that mimic the intestinal crypt and villus, respectively, has allowed studies of multiple enterocyte populations, including a large population of enterocytes that are transitioning from the crypt to the villus. This population expresses NHE3, DRA, and CFTR, representing a combination of Na absorptive and anion secretory functions. In this cell population, these three transporters physically interact, which affects their baseline and regulated activities. A study of this cell population and differentiated Caco-2 cells transduced with NHE3 and endogenously expressing DRA and CFTR has allowed an understanding of previous studies in which cAMP seemed to stimulate and inhibit DRA at the same time. Understanding the contributions of these cells to overall intestinal transport function as part of the fasting and post-prandial state and their contribution to the pathophysiology of diarrheal diseases and some conditions with constipation will allow new approaches to drug development.
Identifiants
pubmed: 35928564
doi: 10.3389/fphys.2022.892112
pii: 892112
pmc: PMC9343792
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
892112Informations de copyright
Copyright © 2022 Donowitz, Sarker, Lin, McNamara, Tse and Singh.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Exp Biol. 2009 Jun;212(Pt 11):1638-46
pubmed: 19448074
Curr Top Membr. 2014;73:69-148
pubmed: 24745981
JOP. 2001 Jul;2(4 Suppl):243-6
pubmed: 11875266
Cell Mol Gastroenterol Hepatol. 2018 Feb 09;5(4):591-609
pubmed: 29930980
J Cell Sci. 2004 Jul 1;117(Pt 15):3353-65
pubmed: 15226406
J Biol Chem. 2017 May 19;292(20):8279-8290
pubmed: 28283572
J Physiol. 2004 May 1;556(Pt 3):791-804
pubmed: 14978207
J Biol Chem. 1998 Aug 14;273(33):20828-36
pubmed: 9694828
Ann N Y Acad Sci. 2009 May;1165:240-8
pubmed: 19538312
Am J Physiol Cell Physiol. 2011 Apr;300(4):C771-82
pubmed: 21191106
Am J Physiol Cell Physiol. 2014 Jul 1;307(1):C55-65
pubmed: 24760985
J Biol Chem. 2009 Aug 28;284(35):23708-18
pubmed: 19535329
J Biol Chem. 2011 Oct 7;286(40):34486-96
pubmed: 21799002
Am J Physiol Cell Physiol. 2013 Aug 1;305(3):C266-75
pubmed: 23703528
J Biol Chem. 2000 Oct 13;275(41):31601-8
pubmed: 10866993
Am J Physiol Renal Physiol. 2016 Aug 1;311(2):F343-51
pubmed: 27279487
Mol Biol Cell. 2008 Sep;19(9):3859-70
pubmed: 18614797
Nat Rev Gastroenterol Hepatol. 2016 Nov;13(11):633-642
pubmed: 27677718
Gastroenterology. 2016 Mar;150(3):638-649.e8
pubmed: 26677983
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3010-5
pubmed: 9096337
Nature. 2009 May 14;459(7244):262-5
pubmed: 19329995
Am J Physiol Cell Physiol. 2002 Sep;283(3):C927-40
pubmed: 12176749
Am J Physiol Gastrointest Liver Physiol. 2009 Feb;296(2):G202-10
pubmed: 19056765
J Gen Physiol. 2006 May;127(5):511-24
pubmed: 16606687
Biochemistry. 2000 May 23;39(20):6123-9
pubmed: 10821685
Cell Mol Gastroenterol Hepatol. 2019;7(3):641-653
pubmed: 30659943
Gastroenterology. 2011 Nov;141(5):1762-72
pubmed: 21889923
J Biol Chem. 2012 Apr 13;287(16):13442-56
pubmed: 22371496
Novartis Found Symp. 2006;273:177-86; discussion 186-92, 261-4
pubmed: 17120768
Nat Cell Biol. 2004 Apr;6(4):343-50
pubmed: 15048129
Physiol Rev. 2019 Oct 1;99(4):2015-2113
pubmed: 31507243
Physiol Rev. 2007 Jul;87(3):825-72
pubmed: 17615390
Cell Physiol Biochem. 2008;22(5-6):693-704
pubmed: 19088451
Curr Opin Gastroenterol. 2009 Mar;25(2):87-91
pubmed: 19528875
J Biol Chem. 2014 Jul 18;289(29):20039-53
pubmed: 24867958
J Clin Invest. 2003 Apr;111(7):931-43
pubmed: 12671039
J Biol Chem. 2002 Dec 27;277(52):50503-9
pubmed: 12403779
J Biol Chem. 2014 Feb 28;289(9):5449-61
pubmed: 24398676
J Biol Chem. 1998 Apr 10;273(15):8790-8
pubmed: 9535857
Annu Rev Physiol. 2005;67:411-43
pubmed: 15709964
Curr Opin Gastroenterol. 2012 Mar;28(2):130-4
pubmed: 22157512
Mol Biol Cell. 2017 Jul 1;28(13):1754-1767
pubmed: 28495796
Mol Biol Cell. 2006 Jun;17(6):2661-73
pubmed: 16540524
Mol Aspects Med. 2013 Apr-Jun;34(2-3):236-51
pubmed: 23506868
J Clin Invest. 2015 Sep;125(9):3519-31
pubmed: 26258413
Cell Mol Gastroenterol Hepatol. 2018 Mar 21;5(4):642-644
pubmed: 29713666
J Physiol. 2005 Aug 15;567(Pt 1):3-11
pubmed: 15905209
Annu Rev Physiol. 2017 Feb 10;79:291-312
pubmed: 28192061