Pericentrin interacts with Kinesin-1 to drive centriole motility.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 09 2022
05 09 2022
Historique:
received:
18
12
2021
revised:
02
06
2022
accepted:
12
07
2022
entrez:
5
8
2022
pubmed:
6
8
2022
medline:
10
8
2022
Statut:
ppublish
Résumé
Centrosome positioning is essential for their function. Typically, centrosomes are transported to various cellular locations through the interaction of centrosomal microtubules (MTs) with motor proteins anchored at the cortex or the nuclear surface. However, it remains unknown how centrioles migrate in cellular contexts in which they do not nucleate MTs. Here, we demonstrate that during interphase, inactive centrioles move directly along the interphase MT network as Kinesin-1 cargo. We identify Pericentrin-Like-Protein (PLP) as a novel Kinesin-1 interacting molecule essential for centriole motility. In vitro assays show that PLP directly interacts with the cargo binding domain of Kinesin-1, allowing PLP to migrate on MTs. Binding assays using purified proteins revealed that relief of Kinesin-1 autoinhibition is critical for its interaction with PLP. Finally, our studies of neural stem cell asymmetric divisions in the Drosophila brain show that the PLP-Kinesin-1 interaction is essential for the timely separation of centrioles, the asymmetry of centrosome activity, and the age-dependent centrosome inheritance.
Identifiants
pubmed: 35929834
pii: 213380
doi: 10.1083/jcb.202112097
pmc: PMC9361567
pii:
doi:
Substances chimiques
Antigens
0
Calmodulin-Binding Proteins
0
Drosophila Proteins
0
Plp protein, Drosophila
0
pericentrin
0
Khc protein, Drosophila
EC 3.6.1.-
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Intramural NIH HHS
ID : ZIA HL006126
Pays : United States
Organisme : NIH HHS
ID : 1ZIAHL006126
Pays : United States
Informations de copyright
This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
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