Identification of novel salt tolerance-associated proteins from the secretome of Enterococcus faecalis.
Enterococcus faecalis
LC–MS/MS
Salt tolerance
Secretome
Structure–function relationship
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
08 Aug 2022
08 Aug 2022
Historique:
received:
01
03
2022
accepted:
04
07
2022
entrez:
7
8
2022
pubmed:
8
8
2022
medline:
10
8
2022
Statut:
epublish
Résumé
The ability of bacteria to adapt to the external environment is fundamental for their survival. A halotolerant microorganism Enterococcus faecalis able to grow under high salt stress conditions was isolated in the present study. The SDS-PAGE analysis of the secretome showed a protein band with a molecular weight of 28 kDa, gradually increased with an increase in salt concentration, and the highest intensity was observed at 15% salt stress condition. LC-MS/MS analysis of this particular band identified fourteen different proteins, out of which nine proteins were uncharacterized. Further, the function of uncharacterized proteins was predicted based on structure-function relationship using a reverse template search approach deciphering uncharacterized protein into type III polyketide synthases, stress-induced protein-1, Eed-h3k79me3, ba42 protein, 3-methyladenine DNA glycosylase, Atxa protein, membrane-bound respiratory hydrogenase, type-i restriction-modification system methylation subunit and ManxA. STRING network analysis further a showed strong association among the proteins. The processes predicted involvement of these proteins in signal transduction, ions transport, synthesis of the protective layer, cellular homeostasis and regulation of gene expression and different metabolic pathways. Thus, the fourteen proteins identified in the secretome play an essential role in maintaining cellular homeostasis in E. faecalis under high-salinity stress. This may represent a novel and previously unreported strategy by E. faecalis to maintain their normal growth and physiology under high salinity conditions.
Identifiants
pubmed: 35934729
doi: 10.1007/s11274-022-03354-w
pii: 10.1007/s11274-022-03354-w
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
177Subventions
Organisme : Indian Council of Agricultural Research
ID : NAIP/Comp-4/C4/C-30033
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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