Construction, expression and functional characterization of an engineered antibody against tumor antigen MUC-1C.
Breast cancer
Engineered antibody
Mammalian expression
Minibody
Mucin-1
Periplasmic expression
Journal
Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
12
04
2022
revised:
02
07
2022
accepted:
20
07
2022
pubmed:
9
8
2022
medline:
30
8
2022
entrez:
8
8
2022
Statut:
ppublish
Résumé
Minibodies (single-chain Fv-CH3) are fusion proteins of a single-chain variable fragment (scFv) to the human IgG1 CH3 domain. They exhibit superior properties as compared to whole antibodies due to their smaller size and less complex composition, and also as compared to scFvs due to the two antigen-binding domains, for immunotherapy and imaging of various carcinomas including breast cancer. In the current study, efficient production of the recombinant anti-MUC-1 minibody for its dominant format (VH-VL) was obtained in the periplasmic space of the Escherichia coliBL21 (DE3) expression system. The active recombinant protein was successfully purified from soluble fraction. Functional assays presented the in vitro targeting properties and specificity of the expressed anti-MUC-1 HL minibody in the MUC-1 positive cell lines compared to normal cell.
Identifiants
pubmed: 35940518
pii: S1046-5928(22)00105-X
doi: 10.1016/j.pep.2022.106148
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antigens, Neoplasm
0
Recombinant Proteins
0
Single-Chain Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106148Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.