Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle.

Animals Fluorescent Antibody Technique Mice Muscle, Skeletal / physiology Satellite Cells, Skeletal Muscle / physiology Staining and Labeling
Antibody Microscopy Model Organisms Stem Cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 9 8 2022
pubmed: 10 8 2022
medline: 11 8 2022
Statut: epublish

Résumé

We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022a).

Identifiants

DOI: 10.1016/j.xpro.2022.101593 PMID: 35942341 PMC: PMC9356158
pubmed: 35942341
doi: 10.1016/j.xpro.2022.101593
pii: S2666-1667(22)00473-7
pmc: PMC9356158
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101593

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Nat Cell Biol. 2010 Feb;12(2):143-52
pubmed: 20081842
Cell Stem Cell. 2022 Feb 3;29(2):265-280.e6
pubmed: 34856120
STAR Protoc. 2022 Apr 11;3(2):101307
pubmed: 35463471

Auteurs

Tamaki Kurosawa (T)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan; Laboratory of Veterinary Pharmacology, Department of Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, Tokyo University, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Madoka Ikemoto-Uezumi (M)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

Akihiro Kaneshige (A)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan; Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan; Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

So-Ichiro Fukada (SI)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Akiyoshi Uezumi (A)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. Electronic address: uezumi.akiyoshi@tokushima-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Whole-mount immunofluorescence staining of...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Technique d'immunofluorescence › Whole-mount immunofluorescence staining of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Whole-mount immunofluorescence staining of...
questionsmedicales.fr › Tissus › Muscles › Muscle strié › Muscles squelettiques › Whole-mount immunofluorescence staining of...
questionsmedicales.fr › Cellules › Cellules souches › Myoblastes › Myoblastes squelettiques › Cellules satellites du muscle squelettique › Whole-mount immunofluorescence staining of...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Whole-mount immunofluorescence staining of...