Changes in the Vaginal Microbiota of Women With Secondary Localized Provoked Vulvodynia.
Journal
Journal of lower genital tract disease
ISSN: 1526-0976
Titre abrégé: J Low Genit Tract Dis
Pays: United States
ID NLM: 9704963
Informations de publication
Date de publication:
01 Oct 2022
01 Oct 2022
Historique:
pubmed:
10
8
2022
medline:
24
9
2022
entrez:
9
8
2022
Statut:
ppublish
Résumé
The etiology of localized provoked vulvodynia (LPV) remains unknown, but observations suggest the involvement of the vaginal microbiota. We examined the vaginal microbiota of women with LPV and healthy controls, upon after a low-oxalate diet (LOD). A total of 9 women diagnosed with secondary LPV and 21 healthy controls were recruited from the Galilee Medical Center in Israel and subjected to prospective evaluations of their vaginal microbiota. Total DNA was extracted from vaginal discharge samples provided before and after following LOD for 3 weeks and was then subjected to 16S sequencing. Data obtained were then used to evaluate α and β diversity, identify differentially abundant bacterial taxa in LPV, and determine their impact on the metabolism. These evaluations revealed decreased diversity in the vaginal microbiota of women with LPV and identified the Ochrobactrum genus and Pseudomonadaceae family as indicators for LPV. In addition, we identified 23 differentially expressed bacterial metabolic pathways between the LPV and control samples and revealed that LOD could induce changes in the β diversity of LPV vaginal microbiomes, which was further supported by some degree of pain reduction in patients. Localized provoked vulvodynia and LOD were associated with shifts in the vaginal microbiota. However, the impact of these changes on the development of LPV requires additional studies with a larger cohort.
Identifiants
pubmed: 35943448
doi: 10.1097/LGT.0000000000000689
pii: 00128360-202210000-00010
doi:
Substances chimiques
Oxalates
0
Banques de données
ClinicalTrials.gov
['NCT02393911']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339-344Informations de copyright
Copyright © 2022, ASCCP.
Déclaration de conflit d'intérêts
The authors have declared they have no conflicts of interest.
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