Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity.
Antioxidant activity
Cell culture condition
Pigments
Rhodobacter sphaeroides
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
15
05
2022
accepted:
11
07
2022
pubmed:
10
8
2022
medline:
11
1
2023
entrez:
9
8
2022
Statut:
ppublish
Résumé
The photosynthetic bacterium, Rhodobacter sphaeroides, is a bacterium that can grow in a variety of environments and produces substances with antioxidant effects. In this study, we investigated the culture conditions to increase the production of antioxidants in R. sphaeroides, which can grow under both aerobic and anaerobic conditions. After incubation in the exponential phase and stationary phase under both conditions, a 2,2-diphenyl-1-picrylhydrazyl assay was used to confirm the antioxidant effect. Although the highest antioxidant effect was shown in the stationary phase under aerobic conditions, the antioxidant effect of each cell was found to be greater when cultured under anaerobic conditions. The antioxidant activity of R. sphaeroides was increased by sonication. In addition, the contents of carotenoids and bacteriochlorophyll, which are pigments with antioxidant effects, produced by R. sphaeroides were measured. We confirmed that the content of carotenoids was higher in anaerobic conditions than in aerobic conditions. However, when measuring the content of the bacterium, we found that there was more content in aerobic conditions. Therefore, we confirm that when grown in anaerobic conditions, the antioxidant effect of R. sphaeroides is higher, which suggests that this antioxidant effect comes from the effect of carotenoid.
Identifiants
pubmed: 35945473
doi: 10.1007/s12033-022-00547-4
pii: 10.1007/s12033-022-00547-4
doi:
Substances chimiques
Antioxidants
0
Carotenoids
36-88-4
Bacteriochlorophylls
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-135Subventions
Organisme : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET)
ID : 321108-04
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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