Evaluation of cancer stem cells markers expression in HCC trough real-time polymerase chain reaction.
CD133
CD44
CD47
Cancer stem cells
EpCAM
Hepatocellular carcinoma
OCT4
Quantitative real-time polymerase chain reaction
Journal
Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
11
8
2022
pubmed:
12
8
2022
medline:
16
8
2022
Statut:
ppublish
Résumé
Quantitative real-time polymerase chain reaction (qRT-PCR) flexibility, robustness and reproducibility have rapidly extended the scope of the method. Cancer stem cells are gaining increasing importance since their role in cancer initiation, treatment resistance and recurrence give rise to a wide range of potential diagnostic and therapeutic applications. The expression of several characteristic markers is proven a reliable method to assess stem-like-phenotype of cancer cells. Here, we provided a thorough protocol for the study of cancer stem cells in hepatocellular carcinoma mouse models and cell cultures using qRT-PCR.
Identifiants
pubmed: 35953204
pii: S0091-679X(22)00050-4
doi: 10.1016/bs.mcb.2022.04.003
pii:
doi:
Substances chimiques
AC133 Antigen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23-32Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Disclosures B.B., E.F., M.G., J.B. and G.M. have no conflicts of interest to disclose.