Identification of Quiescent LGR5

Humans Mice Animals Receptors, G-Protein-Coupled / genetics Stem Cells / metabolism Colon / metabolism Intestinal Mucosa / metabolism Fluorouracil Transforming Growth Factors / metabolism
Intestinal Stem Cell Organoids Slow-Cycling Stem Cell TGF-β Signaling

Journal

Gastroenterology
ISSN: 1528-0012
Titre abrégé: Gastroenterology
Pays: United States
ID NLM: 0374630

Informations de publication

Date de publication:
11 2022
Historique:
received: 13 10 2021
revised: 16 07 2022
accepted: 19 07 2022
pubmed: 14 8 2022
medline: 26 10 2022
entrez: 13 8 2022
Statut: ppublish

Résumé

In the mouse intestinal epithelium, Lgr5 Transcriptional heterogeneity among colonic epithelial cells was analyzed by means of single-cell RNA sequencing analysis of human and mouse colonic epithelial cells. To trace the fate of human colonic stem or differentiated cells, we generated LGR5-tdTomato, LGR5-iCasase9-tdTomato, LGR5-split-Cre, and KRT20-ERCreER knock-in human colon organoids via genome engineering. p27 Single-cell RNA sequencing analysis illuminated the presence of nondividing LGR5 Our results highlight the quiescent nature of human LGR5

Sections du résumé

BACKGROUND & AIMS
In the mouse intestinal epithelium, Lgr5
METHODS
Transcriptional heterogeneity among colonic epithelial cells was analyzed by means of single-cell RNA sequencing analysis of human and mouse colonic epithelial cells. To trace the fate of human colonic stem or differentiated cells, we generated LGR5-tdTomato, LGR5-iCasase9-tdTomato, LGR5-split-Cre, and KRT20-ERCreER knock-in human colon organoids via genome engineering. p27
RESULTS
Single-cell RNA sequencing analysis illuminated the presence of nondividing LGR5
CONCLUSIONS
Our results highlight the quiescent nature of human LGR5

Identifiants

DOI: 10.1053/j.gastro.2022.07.081 PMID: 35963362
pubmed: 35963362
pii: S0016-5085(22)00912-X
doi: 10.1053/j.gastro.2022.07.081
pii:
doi:

Substances chimiques

Receptors, G-Protein-Coupled 0
Fluorouracil U3P01618RT
Transforming Growth Factors 76057-06-2
LGR5 protein, human 0
Lgr5 protein, mouse 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1391-1406.e24

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Keiko Ishikawa (K)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan; Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.

Shinya Sugimoto (S)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan; Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.

Mayumi Oda (M)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Masayuki Fujii (M)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Sirirat Takahashi (S)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Yuki Ohta (Y)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Ai Takano (A)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Kazuhiro Ishimaru (K)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Mami Matano (M)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Kosuke Yoshida (K)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan; Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.

Hikaru Hanyu (H)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Kohta Toshimitsu (K)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Kazuaki Sawada (K)

Center for Integrated Medical Research, School of Medicine, Keio University, Tokyo, Japan.

Mariko Shimokawa (M)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan.

Megumu Saito (M)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan; Fujii Memorial Research Institute, Otsuka Pharmaceutical Company, Limited, Shiga, Japan.

Kenta Kawasaki (K)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan; Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.

Ryota Ishii (R)

Department of Biostatistics, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.

Koji Taniguchi (K)

Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan; Department of Pathology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Takeshi Imamura (T)

Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Ehime, Japan.

Takanori Kanai (T)

Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan.

Toshiro Sato (T)

Department of Organoid Medicine, Sakaguchi Laboratory, Keio University School of Medicine, Tokyo, Japan. Electronic address: t.sato@keio.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Identification of Quiescent LGR5
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Identification of Quiescent LGR5
questionsmedicales.fr › Eucaryotes › Animaux › Identification of Quiescent LGR5
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs couplés aux protéines G › Identification of Quiescent LGR5
questionsmedicales.fr › Cellules › Cellules souches › Identification of Quiescent LGR5
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif inférieur › Gros intestin › Côlon › Identification of Quiescent LGR5
questionsmedicales.fr › Tissus › Membranes › Muqueuse › Muqueuse intestinale › Identification of Quiescent LGR5
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrimidines › Pyrimidinones › Uracile › Fluorouracil › Identification of Quiescent LGR5
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Facteurs de croissance transformants › Identification of Quiescent LGR5