Multispecies biofilm behavior and host interaction support the association of Tannerella serpentiformis with periodontal health.
Tannerella species
biofilm composition and architecture
cell adhesion and invasion
immunostimulatory potential
multispecies model biofilm
periodontitis
Journal
Molecular oral microbiology
ISSN: 2041-1014
Titre abrégé: Mol Oral Microbiol
Pays: Denmark
ID NLM: 101524770
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
15
07
2022
received:
13
06
2022
accepted:
09
08
2022
pubmed:
15
8
2022
medline:
22
3
2023
entrez:
14
8
2022
Statut:
ppublish
Résumé
The recently identified bacterium Tannerella serpentiformis is the closest phylogenetic relative of Tannerella forsythia, whose presence in oral biofilms is associated with periodontitis. Conversely, T. serpentiformis is considered health-associated. This discrepancy was investigated in a comparative study of the two Tannerella species. The biofilm behavior was analyzed upon their addition and of Porphyromonas gingivalis-each bacterium separately or in combinations-to an in vitro five-species oral model biofilm. Biofilm composition and architecture was analyzed quantitatively using real-time PCR and qualitatively by fluorescence in situ hybridization/confocal laser scanning microscopy, and by scanning electron microscopy. The presence of T. serpentiformis led to a decrease of the total cell number of biofilm bacteria, while P. gingivalis was growth-promoting. This effect was mitigated by T. serpentiformis when added to the biofilm together with P. gingivalis. Notably, T. serpentiformis outcompeted T. forsythia numbers when the two species were simultaneously added to the biofilm compared to biofilms containing T. forsythia alone. Tannerella serpentiformis appeared evenly distributed throughout the multispecies biofilm, while T. forsythia was surface-located. Adhesion and invasion assays revealed that T. serpentiformis was significantly less effective in invading human gingival epithelial cells than T. forsythia. Furthermore, compared to T. forsythia, a higher immunostimulatory potential of human gingival fibroblasts and macrophages was revealed for T. serpentiformis, based on mRNA expression levels of the inflammatory mediators interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 and tumor necrosis factor α, and production of the corresponding proteins. Collectively, these data support the potential of T. serpentiformis to interfere with biological processes relevant to the establishment of periodontitis.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
115-133Subventions
Organisme : Austrian Science Fund FWF
ID : P 33618
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 34642
Pays : Austria
Informations de copyright
© 2022 The Authors. Molecular Oral Microbiology published by John Wiley & Sons Ltd.
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