Endosome maturation links PI3Kα signaling to lysosome repopulation during basal autophagy.


Journal

The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664

Informations de publication

Date de publication:
04 10 2022
Historique:
revised: 19 07 2022
received: 14 12 2021
accepted: 21 07 2022
pubmed: 16 8 2022
medline: 6 10 2022
entrez: 15 8 2022
Statut: ppublish

Résumé

Autophagy depends on the repopulation of lysosomes to degrade intracellular components and recycle nutrients. How cells co-ordinate lysosome repopulation during basal autophagy, which occurs constitutively under nutrient-rich conditions, is unknown. Here, we identify an endosome-dependent phosphoinositide pathway that links PI3Kα signaling to lysosome repopulation during basal autophagy. We show that PI3Kα-derived PI(3)P generated by INPP4B on late endosomes was required for basal but not starvation-induced autophagic degradation. PI(3)P signals were maintained as late endosomes matured into endolysosomes, and served as the substrate for the 5-kinase, PIKfyve, to generate PI(3,5)P

Identifiants

pubmed: 35968799
doi: 10.15252/embj.2021110398
pmc: PMC9531306
doi:

Substances chimiques

Phosphatidylinositol Phosphates 0
Protein Aggregates 0
Proteins 0
phosphatidylinositol 3-phosphate 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e110398

Informations de copyright

© 2022 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Samuel J Rodgers (SJ)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Emily I Jones (EI)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Senthil Arumugam (S)

Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
European Molecular Biological Laboratory Australia, Monash University, Clayton, VIC, Australia.

Sabryn A Hamila (SA)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Jill Danne (J)

Monash Ramaciotti Centre for Cryo Electron Microscopy, A Node of Microscopy Australia, Monash University, Clayton, VIC, Australia.

Rajendra Gurung (R)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Matthew J Eramo (MJ)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Randini Nanayakkara (R)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Monash Ramaciotti Centre for Cryo Electron Microscopy, A Node of Microscopy Australia, Monash University, Clayton, VIC, Australia.

Georg Ramm (G)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Monash Ramaciotti Centre for Cryo Electron Microscopy, A Node of Microscopy Australia, Monash University, Clayton, VIC, Australia.

Meagan J McGrath (MJ)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Christina A Mitchell (CA)

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

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Classifications MeSH