The non-functional ACE2 isoform, but not the SARS-CoV-2 receptor, is induced as an interferon-stimulated gene, in SARS-CoV-2 infected adults.


Journal

Cytokine
ISSN: 1096-0023
Titre abrégé: Cytokine
Pays: England
ID NLM: 9005353

Informations de publication

Date de publication:
10 2022
Historique:
received: 25 02 2022
revised: 13 07 2022
accepted: 02 08 2022
pubmed: 16 8 2022
medline: 9 9 2022
entrez: 15 8 2022
Statut: ppublish

Résumé

The recently discovered truncated, non-functional, ACE2 transcript (dACE2), but not the full-length ACE2 (f-lACE2), is induced by IFNs in differentiated airway cells. We measured expression of both ACE2 isoforms in SARS-CoV-2 positive and negative subjects, in relation to Interferon-stimulated genes. A significant activation of dACE2 transcript was found, in SARS-CoV-2 positive adults either hospitalized or not, showing a positive correlation with ISG15; f-lACE2 expression was weakly activated and not ISG-related. We confirmed a specific activation of dACE2 transcript in nasopharyngeal cells, related to the mucosal IFN response.

Identifiants

pubmed: 35969900
pii: S1043-4666(22)00206-X
doi: 10.1016/j.cyto.2022.155997
pmc: PMC9365862
pii:
doi:

Substances chimiques

Antiviral Agents 0
Protein Isoforms 0
Interferons 9008-11-1
Peptidyl-Dipeptidase A EC 3.4.15.1
ACE2 protein, human EC 3.4.17.23
Angiotensin-Converting Enzyme 2 EC 3.4.17.23

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

155997

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Giuseppe Oliveto (G)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy. Electronic address: giuseppe.oliveto@uniroma1.it.

Carolina Scagnolari (C)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Federica Frasca (F)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Leonardo Sorrentino (L)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Luigi Matera (L)

Department of Maternal, Infantile and Urological Sciences, "Sapienza" University, Rome, Italy.

Raffaella Nenna (R)

Department of Maternal, Infantile and Urological Sciences, "Sapienza" University, Rome, Italy.

Agnese Viscido (A)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Mirko Scordio (M)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Laura Petrarca (L)

Department of Maternal, Infantile and Urological Sciences, "Sapienza" University, Rome, Italy.

Anna Maria Zicari (A)

Department of Maternal, Infantile and Urological Sciences, "Sapienza" University, Rome, Italy.

Elio Gentilini (E)

Department of Public Health and Infectious Diseases, "Sapienza" University, Rome, Italy.

Gabriella D'Ettorre (G)

Department of Public Health and Infectious Diseases, "Sapienza" University, Rome, Italy.

Giancarlo Ceccarelli (G)

Department of Public Health and Infectious Diseases, "Sapienza" University, Rome, Italy.

Fabio Midulla (F)

Department of Maternal, Infantile and Urological Sciences, "Sapienza" University, Rome, Italy.

Guido Antonelli (G)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

Alessandra Pierangeli (A)

Virology Laboratory, Department of Molecular Medicine, "Sapienza" University, Rome, Italy.

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Classifications MeSH