A practical guide for the analysis, standardization and interpretation of oxygen consumption measurements.
Journal
Nature metabolism
ISSN: 2522-5812
Titre abrégé: Nat Metab
Pays: Germany
ID NLM: 101736592
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
28
10
2021
accepted:
17
06
2022
pubmed:
16
8
2022
medline:
26
8
2022
entrez:
15
8
2022
Statut:
ppublish
Résumé
Measurement of oxygen consumption is a powerful and uniquely informative experimental technique. It can help identify mitochondrial mechanisms of action following pharmacologic and genetic interventions, and characterize energy metabolism in physiology and disease. The conceptual and practical benefits of respirometry have made it a frontline technique to understand how mitochondrial function can interface with-and in some cases control-cell physiology. Nonetheless, an appreciation of the complexity and challenges involved with such measurements is required to avoid common experimental and analytical pitfalls. Here we provide a practical guide to oxygen consumption measurements covering the selection of experimental models and instrumentation, as well as recommendations for the collection, interpretation and normalization of data. These guidelines are provided with the intention of aiding experimental design and enhancing the overall reputability, transparency and reliability of oxygen consumption measurements.
Identifiants
pubmed: 35971004
doi: 10.1038/s42255-022-00619-4
pii: 10.1038/s42255-022-00619-4
pmc: PMC9618452
mid: NIHMS1825548
doi:
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
978-994Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM138003
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK063491
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA092131
Pays : United States
Informations de copyright
© 2022. Springer Nature Limited.
Références
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5422-7
pubmed: 23513224
Methods Enzymol. 1990;192:280-300
pubmed: 2074793
Nature. 2014 Oct 30;514(7524):628-32
pubmed: 25119024
Nat Methods. 2011 Dec 28;9(1):57-63
pubmed: 22205519
Elife. 2017 Sep 13;6:
pubmed: 28901286
Circ Res. 2021 May 14;128(10):1487-1513
pubmed: 33983836
Annu Rev Physiol. 2022 Feb 10;84:381-407
pubmed: 34758268
Nat Med. 2014 Dec;20(12):1427-35
pubmed: 25419710
Biochim Biophys Acta. 2016 Dec;1862(12):2270-2275
pubmed: 27639835
Methods Mol Biol. 2018;1782:31-70
pubmed: 29850993
J Neurosci. 2005 Jan 5;25(1):164-72
pubmed: 15634778
Cell Rep. 2019 Jul 2;28(1):159-171.e4
pubmed: 31269437
Am J Physiol Gastrointest Liver Physiol. 2015 Jul 1;309(1):G1-9
pubmed: 25977509
Mol Cancer Ther. 2014 Apr;13(4):890-901
pubmed: 24523301
Annu Rev Biochem. 2016 Jun 2;85:161-92
pubmed: 27145841
Nat Protoc. 2006;1(6):2563-72
pubmed: 17406510
Am J Physiol Cell Physiol. 2021 Jan 1;320(1):C80-C91
pubmed: 33147057
Physiology (Bethesda). 2011 Jun;26(3):192-205
pubmed: 21670165
Commun Biol. 2020 May 29;3(1):271
pubmed: 32472013
Biochim Biophys Acta. 2015 Feb;1847(2):171-181
pubmed: 25449966
J Vis Exp. 2021 May 3;(171):
pubmed: 33999016
Mol Metab. 2018 Oct;16:150-159
pubmed: 30098928
Mol Cell. 2005 Jul 22;19(2):197-207
pubmed: 16039589
J Biol Chem. 1980 Mar 25;255(6):2457-64
pubmed: 6766937
Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10996-1001
pubmed: 20534480
Elife. 2020 Aug 17;9:
pubmed: 32804083
Curr Protoc Toxicol. 2015 Nov 02;66:25.7.1-25.7.15
pubmed: 26523474
Anal Chem. 2009 Aug 15;81(16):6868-78
pubmed: 19555051
J Neurochem. 2009 May;109(4):1179-91
pubmed: 19519782
Nature. 2013 Apr 11;496(7444):238-42
pubmed: 23535595
Cell Metab. 2018 Apr 3;27(4):869-885.e6
pubmed: 29617645
Immunity. 2012 Jan 27;36(1):68-78
pubmed: 22206904
Cell Death Differ. 2018 Mar;25(3):542-572
pubmed: 29229998
EMBO Mol Med. 2013 Jun;5(6):904-18
pubmed: 23703906
Cell Metab. 2015 Aug 4;22(2):204-6
pubmed: 26073494
Nucleic Acids Res. 2021 Jan 8;49(D1):D1541-D1547
pubmed: 33174596
J Biol Chem. 2005 Aug 12;280(32):28894-902
pubmed: 15932874
Biochim Biophys Acta. 2011 Dec;1807(12):1507-38
pubmed: 22082452
Curr Opin Biotechnol. 2015 Aug;34:189-201
pubmed: 25731751
Biochim Biophys Acta. 2005 Jan 7;1706(1-2):1-11
pubmed: 15620362
Nature. 2020 Oct;586(7831):790-795
pubmed: 32788725
Methods Mol Biol. 2016;1407:41-61
pubmed: 27271893
Nat Metab. 2021 Sep;3(9):1134-1136
pubmed: 34489606
Mol Metab. 2013 Nov 28;3(2):114-23
pubmed: 24634817
Am J Physiol Cell Physiol. 2009 Jul;297(1):C217-25
pubmed: 19439529
Elife. 2018 Jun 12;7:
pubmed: 29893687
Nat Protoc. 2009;4(11):1582-90
pubmed: 19816421
Free Radic Biol Med. 2014 Apr;69:229-38
pubmed: 24486553
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9893-8
pubmed: 8790427
Cell. 2018 Sep 20;175(1):117-132.e21
pubmed: 30197082
Circ Res. 2015 Feb 27;116(5):e28-39
pubmed: 25605649
Elife. 2019 Jul 18;8:
pubmed: 31305240
APL Bioeng. 2018 Sep;2(3):
pubmed: 30105314
PLoS One. 2011 Mar 28;6(3):e18317
pubmed: 21512578
Diabetes. 2013 Apr;62(4):1041-53
pubmed: 23520284
Biochim Biophys Acta. 2012 Oct;1822(10):1570-80
pubmed: 22800932
Nature. 2020 Dec;588(7836):174-179
pubmed: 32906142
Methods Mol Biol. 2018;1782:71-87
pubmed: 29850994
Nat Protoc. 2010 Sep;5(9):1540-51
pubmed: 20725069
Trends Cell Biol. 2017 Sep;27(9):633-644
pubmed: 28522206
Sci Adv. 2020 Oct 21;6(43):
pubmed: 33087354
Exp Gerontol. 2015 Oct;70:84-91
pubmed: 26226578
Biochim Biophys Acta. 2005 Jul 15;1708(3):375-92
pubmed: 15979561
J Immunol Methods. 1981;42(2):195-201
pubmed: 7017006
Front Physiol. 2019 Mar 15;10:213
pubmed: 30930787
Nat Metab. 2019 Jan;1:16-33
pubmed: 31032474
Nat Immunol. 2014 Sep;15(9):846-55
pubmed: 25086775
Biol Chem. 2012 Dec;393(12):1485-1512
pubmed: 23092819
J Biol Chem. 1955 Nov;217(1):409-27
pubmed: 13271404
Biochem J. 2011 Apr 15;435(2):297-312
pubmed: 21726199
Nature. 2016 Apr 14;532(7598):255-8
pubmed: 27049945
STAR Protoc. 2021 Mar 18;2(1):100386
pubmed: 33778780
J Cell Biol. 2017 Jan 2;216(1):31-40
pubmed: 28031422
Methods Enzymol. 2014;547:309-54
pubmed: 25416364
J Clin Invest. 2013 Jan;123(1):469-78
pubmed: 23257354
Trends Biochem Sci. 2017 Oct;42(10):824-843
pubmed: 28927698
Toxicol In Vitro. 2012 Apr;26(3):511-25
pubmed: 22261204
Biochem J. 1992 Apr 1;283 ( Pt 1):41-50
pubmed: 1373604
J Physiol. 2012 Jun 15;590(12):2845-71
pubmed: 22495585
Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16823-7
pubmed: 20847295
Physiol Rev. 1997 Jul;77(3):731-58
pubmed: 9234964
N Engl J Med. 2019 Nov 21;381(21):1995-2008
pubmed: 31535829
PLoS One. 2011;6(7):e21746
pubmed: 21799747
Biochem Soc Trans. 2005 Nov;33(Pt 5):897-904
pubmed: 16246006
Genes Dev. 2011 Aug 15;25(16):1716-33
pubmed: 21852536
J Cell Biol. 2017 Apr 3;216(4):1091-1105
pubmed: 28254829
Biochem Soc Trans. 2009 Dec;37(Pt 6):1385-8
pubmed: 19909281
Nat Immunol. 2013 Oct;14(10):1064-72
pubmed: 23955661
Adv Exp Med Biol. 2016;899:167-96
pubmed: 27325267
Nat Protoc. 2007;2(2):287-95
pubmed: 17406588
Science. 2012 Jul 6;337(6090):96-100
pubmed: 22628558
Cell. 2018 May 3;173(4):822-837
pubmed: 29727671
Nat Rev Drug Discov. 2018 Dec;17(12):865-886
pubmed: 30393373
J Biol Chem. 2017 Apr 28;292(17):7189-7207
pubmed: 28270511
STAR Protoc. 2021 Jul 31;2(3):100687
pubmed: 34401773
Essays Biochem. 2010;47:1-23
pubmed: 20533897
Cell Metab. 2015 Oct 6;22(4):669-81
pubmed: 26344103
JCI Insight. 2019 Apr 16;5:
pubmed: 30990467
Nat Genet. 1997 Oct;17(2):215-7
pubmed: 9326946
PLoS One. 2012;7(4):e34465
pubmed: 22496810
Methods Enzymol. 2014;542:125-49
pubmed: 24862264
Lab Invest. 2013 Jun;93(6):690-700
pubmed: 23528848
Life (Basel). 2021 Sep 10;11(9):
pubmed: 34575097
J Biol Chem. 2021 Oct;297(4):101140
pubmed: 34461088
EMBO Rep. 2020 Dec 3;21(12):e49634
pubmed: 33275313
Mol Cell. 2016 Aug 18;63(4):621-632
pubmed: 27499296
Genes Dev. 2013 Dec 15;27(24):2615-27
pubmed: 24352419
Prog Lipid Res. 2005 Mar-May;44(2-3):125-53
pubmed: 15893380
Free Radic Biol Med. 2010 Apr 1;48(7):905-14
pubmed: 20093177
Anal Biochem. 2018 Jul 1;552:60-65
pubmed: 28987935
FASEB J. 2013 Jun;27(6):2392-406
pubmed: 23475850
Nat Med. 2018 Jul;24(7):1036-1046
pubmed: 29892070
J Cell Biol. 1981 Dec;91(3 Pt 2):227s-255s
pubmed: 7033239
Science. 2012 Jul 6;337(6090):93-6
pubmed: 22628554
Sci Signal. 2014 Jan 28;7(310):ra10
pubmed: 24473195
Invest Ophthalmol Vis Sci. 2015 Dec;56(13):8428-36
pubmed: 26747773
Biochem J. 2006 Jan 1;393(Pt 1):151-9
pubmed: 16137248
Nat Protoc. 2014 Feb;9(2):421-38
pubmed: 24457333
Curr Protoc Toxicol. 2014 May 27;60:25.2.1-16
pubmed: 24865646
Exp Mol Med. 2018 Apr 16;50(4):1-13
pubmed: 29657327
Nat Med. 2014 Jun;20(6):616-23
pubmed: 24813252
J Biol Chem. 2018 May 18;293(20):7522-7530
pubmed: 29191830
Science. 2019 Feb 8;363(6427):582-583
pubmed: 30733403
J Biomol Screen. 2003 Oct;8(5):566-70
pubmed: 14567784
Methods Mol Biol. 2017;1567:15-32
pubmed: 28276010