3D Bioprinted Hydroxyapatite or Graphene Oxide Containing Nanocellulose-Based Scaffolds for Bone Regeneration.


Journal

Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941

Informations de publication

Date de publication:
11 2022
Historique:
revised: 26 07 2022
received: 09 06 2022
pubmed: 19 8 2022
medline: 16 11 2022
entrez: 18 8 2022
Statut: ppublish

Résumé

Bone tissue is usually damaged after big traumas, tumors, and increasing aging-related diseases such as osteoporosis and osteoarthritis. Current treatments are based on implanting grafts, which are shown to have several inconveniences. In this regard, tissue engineering through the 3D bioprinting technique has arisen to manufacture structures that would be a feasible therapeutic option for bone regenerative medicine. In this study, nanocellulose-alginate (NC-Alg)-based bioink is improved by adding two different inorganic components such as hydroxyapatite (HAP) and graphene oxide (GO). First, ink rheological properties and biocompatibility are evaluated as well as the influence of the sterilization process on them. Then, scaffolds are characterized. Finally, biological studies of embedded murine D1 mesenchymal stem cells engineered to secrete erythropoietin are performed. Results show that the addition of both HAP and GO prevents NC-Alg ink from viscosity lost in the sterilization process. However, GO is reduced due to short cycle autoclave sterilization, making it incompatible with this ink. In addition, HAP and GO have different influences on scaffold architecture and surface as well as in swelling capacity. Scaffolds mechanics, as well as cell viability and functionality, are promoted by both elements addition. Additionally, GO demonstrates an enhanced bone differentiation capacity.

Identifiants

pubmed: 35981208
doi: 10.1002/mabi.202200236
doi:

Substances chimiques

Durapatite 91D9GV0Z28
graphene oxide 0
Alginates 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2200236

Informations de copyright

© 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.

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Auteurs

Markel Lafuente-Merchan (M)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

Sandra Ruiz-Alonso (S)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

Fátima García-Villén (F)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

Alaitz Zabala (A)

Mechanical and Industrial Manufacturing Department, Mondragon Unibertsitatea, Loramendi 4, Mondragón, 20500, Spain.

Ana M Ochoa de Retana (AMO)

Department of Organic Chemistry I, Faculty of Pharmacy and Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, Vitoria, 01006, Spain.

Idoia Gallego (I)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

Laura Saenz-Del-Burgo (L)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

Jose Luis Pedraz (JL)

NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU)., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Health Institute Carlos III., Paseo de la Universidad 7, Vitoria-Gasteiz, 01006, Spain.
Bioaraba, NanoBioCel Resarch Group, Vitoria-Gasteiz, 01009, Spain.

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