Proteomic Analysis of Murine Bone Marrow Very Small Embryonic-like Stem Cells at Steady-State Conditions and after In Vivo Stimulation by Nicotinamide and Follicle-Stimulating Factor Reflects their Germ-Lineage Origin and Multi Germ Layer Differentiation Potential.
FSH
Germ line commitment
Nicotinamide
PGCs
Proteomics
UN-SDG3
VSELs
Journal
Stem cell reviews and reports
ISSN: 2629-3277
Titre abrégé: Stem Cell Rev Rep
Pays: United States
ID NLM: 101752767
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
accepted:
09
08
2022
pubmed:
20
8
2022
medline:
11
1
2023
entrez:
19
8
2022
Statut:
ppublish
Résumé
Very small embryonic-like stem cells (VSELs) are a dormant population of development early stem cells deposited in adult tissues that as demonstrated contribute to tissue/organ repair and regeneration. We postulated developmental relationship of these cells to migrating primordial germ cells (PGCs) and explained the quiescent state of these cells by the erasure of differently methylated regions (DMRs) at some of the paternally imprinted genes involved in embryogenesis. Recently, we reported that VSELs began to proliferate and expand in vivo in murine bone marrow (BM) after exposure to nicotinamide (NAM) and selected pituitary and gonadal sex hormones. In the current report, we performed proteomic analysis of VSELs purified from murine bone marrow (BM) after repeated injections of NAM + Follicle-Stimulating Hormone (FSH) that in our previous studies turned out to be an effective combination to expand these cells. By employing the Gene Ontology (GO) resources, we have performed a combination of standard GO annotations (GO-CAM) to produce a network between BM steady-state conditions VSELs (SSC-VSELS) and FSH + NAM expanded VSELs (FSH + NAM VSELs). We have identified several GO biological processes regulating development, organogenesis, gene expression, signal transduction, Wnt signaling, insulin signaling, cytoskeleton organization, cell adhesion, inhibiting apoptosis, responses to extra- and intracellular stimuli, protein transport and stabilization, protein phosphorylation and ubiquitination, DNA repair, immune response, and regulation of circadian rhythm. We report that VSELs express a unique panel of proteins that only partially overlapped with the proteome of BM - derived hematopoietic stem cells (HSCs) and hematopoietic mononuclear cells (MNCs) and respond to FSH + NAM stimulation by expressing proteins involved in the development of all three germ layers. Thus, our current data supports further germ-lineage origin and multi germ layer differentiation potential of these cells.
Identifiants
pubmed: 35986128
doi: 10.1007/s12015-022-10445-6
pii: 10.1007/s12015-022-10445-6
pmc: PMC9823037
doi:
Substances chimiques
Follicle Stimulating Hormone
9002-68-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
120-132Informations de copyright
© 2022. The Author(s).
Références
Int J Dev Biol. 2010;54(6-7):1151-63
pubmed: 20711992
Leukemia. 2010 Aug;24(8):1450-61
pubmed: 20508611
Nat Commun. 2012 May 29;3:859
pubmed: 22643886
Cell Rep. 2017 Feb 21;18(8):1930-1945
pubmed: 28228259
Stem Cells Dev. 2012 Jul 1;21(10):1639-52
pubmed: 22023227
Blood. 2008 Feb 1;111(3):1543-51
pubmed: 17940206
J Cell Mol Med. 2011 Jun;15(6):1319-28
pubmed: 20629987
Stem Cells Dev. 2014 Apr 1;23(7):689-701
pubmed: 24372153
Cells. 2021 Jun 22;10(7):
pubmed: 34206516
Circ Res. 2019 Jan 18;124(2):208-210
pubmed: 30653438
Stem Cell Rev Rep. 2018 Feb;14(1):110-124
pubmed: 29168113
Lab Invest. 2006 Jul;86(7):654-63
pubmed: 16652109
Leukemia. 2022 Jan;36(1):23-32
pubmed: 34853440
Reprod Biol Endocrinol. 2015 Apr 18;13:33
pubmed: 25903688
PLoS One. 2010 Aug 10;5(8):e12072
pubmed: 20711465
Leukemia. 2015 Apr;29(4):776-82
pubmed: 25486871
Curr Top Dev Biol. 2011;97:21-53
pubmed: 22074601
Blood. 2001 Feb 15;97(4):1099-105
pubmed: 11159543
Genes Dev. 2005 Jun 1;19(11):1376-89
pubmed: 15937223
J Cell Mol Med. 2008 Jan-Feb;12(1):292-303
pubmed: 18031297
Differentiation. 2008 Oct;76(8):843-56
pubmed: 18452550
Stem Cells Dev. 2014 Apr 1;23(7):702-13
pubmed: 24299281
Front Immunol. 2021 Feb 25;12:629986
pubmed: 33717157
Microsc Res Tech. 2017 Apr;80(4):430-440
pubmed: 27990704
Nat Cell Biol. 2015 Jun;17(6):771-81
pubmed: 25961503
Genetics. 2013 Jun;194(2):447-57
pubmed: 23608191
Leukemia. 2006 May;20(5):857-69
pubmed: 16498386
Stem Cell Rev Rep. 2018 Oct;14(5):715-721
pubmed: 29876729
Stroke. 2009 Apr;40(4):1237-44
pubmed: 19246697
J Ovarian Res. 2021 Oct 30;14(1):145
pubmed: 34717703
Transl Cancer Res. 2019 Dec;8(8):2916-2923
pubmed: 35117049
Leukemia. 2009 Nov;23(11):2042-51
pubmed: 19641521
Mol Immunol. 2017 Sep;89:2-9
pubmed: 28601357
Science. 2018 Dec 7;362(6419):1161-1164
pubmed: 30409806
Stem Cells. 2013 Dec;31(12):2759-66
pubmed: 23681901
Reprod Biol Endocrinol. 2017 Nov 16;15(1):89
pubmed: 29145898
Cell J. 2016 Winter;17(4):639-47
pubmed: 26862523
J Cell Physiol. 2015 Aug;230(8):1852-61
pubmed: 25545634
Stem Cells Dev. 2016 Jan 1;25(1):27-42
pubmed: 26595762
Genes Dev. 2002 Oct 1;16(19):2509-17
pubmed: 12368262
Cell Biol Int. 2012;36(12):1185-93
pubmed: 22988836
Circ Res. 2017 Jan 6;120(1):166-178
pubmed: 28057792
Front Cell Dev Biol. 2021 Jan 15;8:590009
pubmed: 33520982
Thromb Haemost. 2015 May;113(5):1084-94
pubmed: 25608764
Stem Cells Dev. 2014 Nov 1;23(21):2661-71
pubmed: 24914588
Stem Cells Dev. 2015 Apr 15;24(8):927-37
pubmed: 25607657
Am J Physiol Heart Circ Physiol. 2013 Nov 1;305(9):H1354-62
pubmed: 23997098
Leukemia. 2013 Apr;27(4):773-9
pubmed: 23135355
Stem Cells Dev. 2012 May 20;21(8):1367-79
pubmed: 22032240
J Cell Physiol. 2014 Oct;229(10):1466-74
pubmed: 24648201
Mol Biol Cell. 2015 Apr 1;26(7):1225-37
pubmed: 25657325
Leukemia. 2014 Mar;28(3):473-84
pubmed: 24018851
Nat Neurosci. 2012 Sep;15(9):1201-10
pubmed: 22842144
Leukemia. 2015 Sep;29(9):1909-17
pubmed: 25882698
J Cell Physiol. 2010 Jan;222(1):50-6
pubmed: 19813271
Exp Hematol. 2011 Feb;39(2):225-37
pubmed: 21034791
Int J Oncol. 2010 Aug;37(2):237-47
pubmed: 20596650