Differential expression of glycans in the urothelial layers of horse urinary bladder.
Fucose
Glycocalyx
Glycohistochemistry
Horse
Lectin
Secretion
Sialic acid
Sugar
Urothelium
Journal
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
ISSN: 1618-0402
Titre abrégé: Ann Anat
Pays: Germany
ID NLM: 100963897
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
received:
05
04
2022
revised:
26
07
2022
accepted:
02
08
2022
pubmed:
21
8
2022
medline:
26
10
2022
entrez:
20
8
2022
Statut:
ppublish
Résumé
Urothelium is a multilayer epithelium covering the inner surface of the urinary bladder that acts as a blood-urine barrier and is involved in maintaining the wellbeing of the whole organism. Glycans serve in the maturation and differentiation of cells and thus play a key role in the morphology and function of the multilayered epithelium. The aim of the present study was to examine the glycoprotein pattern of the horse urinary bladder urothelium by lectin histochemistry. The study involved urinary bladders from four horse stallions. Tissue sections were stained with a panel of eleven lectins, in combination with saponification and sialidase digestion (Ks). Basal cells displayed high-mannose N-glycans (Con A), α2,6-linked sialic acid (SNA), and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA). The young intermediate cells expressed terminal N-acetylglucosamine (GlcNAc) (GSA II), galactose (GSA I-B This study showed that the glycan pattern becomes more complex from the basal to surface layer of the urothelium and that surface cells could modify the composition of urine via the secretion of glycoproteins.
Sections du résumé
BACKGROUND
BACKGROUND
Urothelium is a multilayer epithelium covering the inner surface of the urinary bladder that acts as a blood-urine barrier and is involved in maintaining the wellbeing of the whole organism. Glycans serve in the maturation and differentiation of cells and thus play a key role in the morphology and function of the multilayered epithelium. The aim of the present study was to examine the glycoprotein pattern of the horse urinary bladder urothelium by lectin histochemistry.
METHODS
METHODS
The study involved urinary bladders from four horse stallions. Tissue sections were stained with a panel of eleven lectins, in combination with saponification and sialidase digestion (Ks).
RESULTS
RESULTS
Basal cells displayed high-mannose N-glycans (Con A), α2,6-linked sialic acid (SNA), and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA). The young intermediate cells expressed terminal N-acetylglucosamine (GlcNAc) (GSA II), galactose (GSA I-B
CONCLUSIONS
CONCLUSIONS
This study showed that the glycan pattern becomes more complex from the basal to surface layer of the urothelium and that surface cells could modify the composition of urine via the secretion of glycoproteins.
Identifiants
pubmed: 35987426
pii: S0940-9602(22)00103-0
doi: 10.1016/j.aanat.2022.151988
pii:
doi:
Substances chimiques
Mannose
PHA4727WTP
N-Acetylneuraminic Acid
GZP2782OP0
Neuraminidase
EC 3.2.1.18
Fucose
28RYY2IV3F
Galactose
X2RN3Q8DNE
Acetylgalactosamine
KM15WK8O5T
Acetylglucosamine
V956696549
Polysaccharides
0
Lectins
0
Glycoproteins
0
Mucins
0
Antigens, Viral, Tumor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151988Informations de copyright
Copyright © 2022 Elsevier GmbH. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.