Multiple Viral Protein Genome-Linked Proteins Compensate for Viral Translation in a Positive-Sense Single-Stranded RNA Virus Infection.
5' Untranslated Regions
/ genetics
Animals
Cell Line
Dicistroviridae
/ genetics
Drosophila
/ cytology
Genome, Viral
/ genetics
Internal Ribosome Entry Sites
/ genetics
Mutation
Protein Biosynthesis
RNA Virus Infections
/ virology
RNA, Viral
/ genetics
Serine
/ metabolism
Threonine
/ metabolism
Viral Load
Viral Proteins
/ biosynthesis
RNA
RNA virus
VPg
dicistrovirus
insect viruses
picornaviruses
polyprotein
replication
virology
virus
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
14 09 2022
14 09 2022
Historique:
pubmed:
23
8
2022
medline:
17
9
2022
entrez:
22
8
2022
Statut:
ppublish
Résumé
Viral protein genome-linked (VPg) protein plays an essential role in protein-primed replication of plus-stranded RNA viruses. VPg is covalently linked to the 5' end of the viral RNA genome via a phosphodiester bond typically at a conserved amino acid. Whereas most viruses have a single VPg, some viruses have multiple VPgs that are proposed to have redundant yet undefined roles in viral replication. Here, we use cricket paralysis virus (CrPV), a dicistrovirus that has four nonidentical copies of VPg, as a model to characterize the role of VPg copies in infection. Dicistroviruses contain two main open reading frames (ORFs) that are driven by distinct internal ribosome entry sites (IRESs). We systematically generated single and combinatorial deletions and mutations of VPg1 to VPg4 within the CrPV infectious clone and monitored viral yield in
Identifiants
pubmed: 35993738
doi: 10.1128/jvi.00699-22
pmc: PMC9472611
doi:
Substances chimiques
5' Untranslated Regions
0
Internal Ribosome Entry Sites
0
RNA, Viral
0
Viral Proteins
0
Threonine
2ZD004190S
Serine
452VLY9402
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0069922Subventions
Organisme : CIHR
ID : PJT-178342
Pays : Canada
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