Transcriptome analysis of Micrococcus luteus in response to treatment with protocatechuic acid.
Micrococcus luteus
antibacterial mechanism
protocatechuic acid
skimmed milk
transcriptome
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
15
07
2022
received:
31
03
2022
accepted:
19
07
2022
pubmed:
24
8
2022
medline:
22
10
2022
entrez:
23
8
2022
Statut:
ppublish
Résumé
To reveal the antibacterial mechanism of protocatechuic acid (PCA) against Micrococcus luteus. M. luteus was exposed to PCA, and the antibacterial mechanism was revealed by measuring membrane potential, intracellular ATP and pH levels and transcriptome analysis. PCA induced the membrane potential depolarization of M. luteus, significantly decreased the intracellular ATP and pH levels of M. luteus and disrupted the integrity of the M. luteus cell membrane. Transcriptome analysis showed that PCA induced 782 differentially expressed genes (DEGs) of M. luteus. GO enrichment analysis revealed that the majority of DEGs are involved in pathways of metabolic process, cellular process, biological regulation and transport activity. In addition, PCA inhibited the growth of M. luteus in skimmed milk and extended the shelf life of skimmed milk. PCA had good bactericidal activity against M. luteus through the mechanism of cell membrane disruption and metabolic process disorder. PCA inhibits the growth of M. luteus in skimmed milk, suggesting that PCA is promising to be used as a novel preservative in food storage.
Substances chimiques
protocatechuic acid
36R5QJ8L4B
Anti-Bacterial Agents
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3139-3149Subventions
Organisme : Xianyang Qin chuangyuan Science and Technology Innovation Project
ID : 2021ZDZX-NY-0007
Organisme : Shaanxi Provincial Education Department
ID : 21JK0541
Organisme : Key Research and Development Projects of Shaanxi Province
ID : 2020NY-108
Organisme : Key Research and Development Projects of Shaanxi Province
ID : 2021NY-124
Informations de copyright
© 2022 Society for Applied Microbiology.
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