Engineered Fluorescent Strains of Cryptococcus neoformans: a Versatile Toolbox for Studies of Host-Pathogen Interactions and Fungal Biology, Including the Viable but Nonculturable State.
Cryptococcus neoformans
GFP
H99
VBNC
dormancy
fluorescently tagged strains
histone H3
mCherry
poly(A) binding protein
stress granules
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
26 10 2022
26 10 2022
Historique:
pubmed:
26
8
2022
medline:
29
10
2022
entrez:
25
8
2022
Statut:
ppublish
Résumé
Cryptococcus neoformans is an opportunistic fungal pathogen known for its remarkable ability to infect and subvert phagocytes. This ability provides survival and persistence within the host and relies on phenotypic plasticity. The viable but nonculturable (VBNC) phenotype was recently described in C. neoformans, whose study is promising in understanding the pathophysiology of cryptococcosis. The use of fluorescent strains is improving host interaction research, but it is still underexploited. Here, we fused histone H3 or the poly(A) binding protein (Pab) to enhanced green fluorescent protein (eGFP) or mCherry, obtaining a set of C. neoformans transformants with different colors, patterns of fluorescence, and selective markers (hygromycin B resistance [Hyg
Identifiants
pubmed: 36005449
doi: 10.1128/spectrum.01504-22
pmc: PMC9603711
doi:
Substances chimiques
Histones
0
Hygromycin B
3XQ2233B0B
Neomycin
I16QD7X297
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0150422Références
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