Designed peptides as nanomolar cross-amyloid inhibitors acting via supramolecular nanofiber co-assembly.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
25 08 2022
Historique:
received: 16 01 2022
accepted: 10 08 2022
entrez: 25 8 2022
pubmed: 26 8 2022
medline: 30 8 2022
Statut: epublish

Résumé

Amyloid self-assembly is linked to numerous devastating cell-degenerative diseases. However, designing inhibitors of this pathogenic process remains a major challenge. Cross-interactions between amyloid-β peptide (Aβ) and islet amyloid polypeptide (IAPP), key polypeptides of Alzheimer's disease (AD) and type 2 diabetes (T2D), have been suggested to link AD with T2D pathogenesis. Here, we show that constrained peptides designed to mimic the Aβ amyloid core (ACMs) are nanomolar cross-amyloid inhibitors of both IAPP and Aβ42 and effectively suppress reciprocal cross-seeding. Remarkably, ACMs act by co-assembling with IAPP or Aβ42 into amyloid fibril-resembling but non-toxic nanofibers and their highly ordered superstructures. Co-assembled nanofibers exhibit various potentially beneficial features including thermolability, proteolytic degradability, and effective cellular clearance which are reminiscent of labile/reversible functional amyloids. ACMs are thus promising leads for potent anti-amyloid drugs in both T2D and AD while the supramolecular nanofiber co-assemblies should inform the design of novel functional (hetero-)amyloid-based nanomaterials for biomedical/biotechnological applications.

Identifiants

pubmed: 36008417
doi: 10.1038/s41467-022-32688-0
pii: 10.1038/s41467-022-32688-0
pmc: PMC9411207
doi:

Substances chimiques

Amyloid 0
Amyloid beta-Peptides 0
Amyloidogenic Proteins 0
Islet Amyloid Polypeptide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5004

Informations de copyright

© 2022. The Author(s).

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Auteurs

Karin Taş (K)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.

Beatrice Dalla Volta (BD)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.

Christina Lindner (C)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.
Institute of Organic Chemistry, Centre for Advanced Materials, 69120, Heidelberg, Germany.

Omar El Bounkari (O)

Division of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilian-University (LMU), 81377, Munich, Germany.

Kathleen Hille (K)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.

Yuan Tian (Y)

Division of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilian-University (LMU), 81377, Munich, Germany.

Xènia Puig-Bosch (X)

Department of Anesthesiology and Intensive Care, Technical University of Munich/Klinikum Rechts der Isar, 81675, München, Germany.

Markus Ballmann (M)

Department of Anesthesiology and Intensive Care, Technical University of Munich/Klinikum Rechts der Isar, 81675, München, Germany.

Simon Hornung (S)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.

Martin Ortner (M)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.
Chair of Biopolymer Chemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.

Sophia Prem (S)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany.
Werner Siemens-Chair of Synthetic Biotechnology (WSSB), Technical University of Munich (TUM), Department of Chemistry, 85748, Garching, Germany.

Laura Meier (L)

Center for Protein Assemblies, Department of Chemistry, Technical University of Munich, 85748, Garching, Germany.

Gerhard Rammes (G)

Department of Anesthesiology and Intensive Care, Technical University of Munich/Klinikum Rechts der Isar, 81675, München, Germany.

Martin Haslbeck (M)

Center for Protein Assemblies, Department of Chemistry, Technical University of Munich, 85748, Garching, Germany.

Christian Weber (C)

Institute for Cardiovascular Prevention, Klinikum der Universität München, Ludwig-Maximilian-University Munich (LMU), 80336, Munich, Germany.
Munich Cluster for Systems Neurology (SyNergy), 81377, Munich, Germany.
German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, 80802, Munich, Germany.
Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 6229, Maastricht, The Netherlands.

Remco T A Megens (RTA)

Institute for Cardiovascular Prevention, Klinikum der Universität München, Ludwig-Maximilian-University Munich (LMU), 80336, Munich, Germany.
German Centre for Cardiovascular Research (DZHK), partner site Munich Heart Alliance, 80802, Munich, Germany.
Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 6229, Maastricht, The Netherlands.

Jürgen Bernhagen (J)

Division of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, Ludwig-Maximilian-University (LMU), 81377, Munich, Germany.
Munich Cluster for Systems Neurology (SyNergy), 81377, Munich, Germany.

Aphrodite Kapurniotu (A)

Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich (TUM), 85354, Freising, Germany. akapurniotu@mytum.de.

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