Alpha-Ketoglutarate or 5-HMF: Single Compounds Effectively Eliminate Leukemia Cells via Caspase-3 Apoptosis and Antioxidative Pathways.
5-hydroxy-methyl-furfural (5-HMF)
alpha-ketoglutarate (aKG)
caspase activity
leukemia
nitrated tyrosine
peroxynitrite (ONOO−)
proliferation
reactive oxygen and nitrogen species (RONS)
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
12 Aug 2022
12 Aug 2022
Historique:
received:
01
07
2022
revised:
31
07
2022
accepted:
08
08
2022
entrez:
26
8
2022
pubmed:
27
8
2022
medline:
30
8
2022
Statut:
epublish
Résumé
We recently showed that a combined solution containing alpha-ketoglutarate (aKG) and 5-hydroxymethyl-furfural (5-HMF) has a solid antitumoral effect on the Jurkat cell line due to the fact of its antioxidative, caspase-3 and apoptosis activities, but no negative effect on human fibroblasts was obtained. The question arises how the single compounds, aKG and 5-HMF, affect peroxynitrite (ONOO The ONOO aKG showed a concentration-dependent reduction in ONOO Both substances showed high antioxidative potential in eliminating either peroxynitrite or nitration of tyrosine residues, which results in a better inhibition of cell growth and mitochondrial activity of 5-HMF compared to aKG. However, caspase-3-activated apoptosis measurements revealed that the combination of both substances synergistically is the most effective compared to single compounds.
Sections du résumé
BACKGROUND
BACKGROUND
We recently showed that a combined solution containing alpha-ketoglutarate (aKG) and 5-hydroxymethyl-furfural (5-HMF) has a solid antitumoral effect on the Jurkat cell line due to the fact of its antioxidative, caspase-3 and apoptosis activities, but no negative effect on human fibroblasts was obtained. The question arises how the single compounds, aKG and 5-HMF, affect peroxynitrite (ONOO
METHODS
METHODS
The ONOO
RESULTS
RESULTS
aKG showed a concentration-dependent reduction in ONOO
CONCLUSION
CONCLUSIONS
Both substances showed high antioxidative potential in eliminating either peroxynitrite or nitration of tyrosine residues, which results in a better inhibition of cell growth and mitochondrial activity of 5-HMF compared to aKG. However, caspase-3-activated apoptosis measurements revealed that the combination of both substances synergistically is the most effective compared to single compounds.
Identifiants
pubmed: 36012295
pii: ijms23169034
doi: 10.3390/ijms23169034
pmc: PMC9409265
pii:
doi:
Substances chimiques
Antioxidants
0
Ketoglutaric Acids
0
Peroxynitrous Acid
14691-52-2
Tyrosine
42HK56048U
Caspase 3
EC 3.4.22.-
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
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