Integrated and automated high-throughput purification of libraries on microscale.
Analytical chemistry automation
High-throughput purification
Laboratory automation
Library purification
Microscale libraries
Journal
SLAS technology
ISSN: 2472-6311
Titre abrégé: SLAS Technol
Pays: United States
ID NLM: 101697564
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
07
03
2022
revised:
04
08
2022
accepted:
21
08
2022
pubmed:
27
8
2022
medline:
15
12
2022
entrez:
26
8
2022
Statut:
ppublish
Résumé
We herein report the development of an automation platform for rapid purification and quantification of chemical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on analytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.
Identifiants
pubmed: 36028206
pii: S2472-6303(22)05172-X
doi: 10.1016/j.slast.2022.08.002
pii:
doi:
Substances chimiques
Small Molecule Libraries
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
350-360Informations de copyright
Copyright © 2022. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.