Single-cell immune repertoire sequencing of B and T cells in murine models of infection and autoimmunity.
Journal
Genes and immunity
ISSN: 1476-5470
Titre abrégé: Genes Immun
Pays: England
ID NLM: 100953417
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
23
02
2022
accepted:
09
08
2022
revised:
04
08
2022
pubmed:
27
8
2022
medline:
1
10
2022
entrez:
26
8
2022
Statut:
ppublish
Résumé
Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. Recent advances in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional information. Here, we recovered transcriptome and immune repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ T cells with binding specificity restricted to two distinct LCMV peptides, and B and T cells isolated from the nervous system in the context of experimental autoimmune encephalomyelitis. We could relate clonal expansion, germline gene usage, and clonal convergence to cell phenotypes spanning activation, memory, naive, antibody secretion, T-cell inflation, and regulation. Together, this dataset provides a resource for immunologists that can be integrated with future single-cell immune repertoire and transcriptome sequencing datasets.
Identifiants
pubmed: 36028771
doi: 10.1038/s41435-022-00180-w
pii: 10.1038/s41435-022-00180-w
pmc: PMC9519453
doi:
Substances chimiques
Peptides
0
Receptors, Antigen, T-Cell
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
183-195Informations de copyright
© 2022. The Author(s).
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